摘要
目的:探讨子宫内膜癌（EC）组织中微小RNA-25-3p（miR-25-3p）、磷酸酶和紧张素同源物（PTEN）的表达及与病理参数的关系。方法:选取2020年1月~2022年11月武汉市妇幼保健院收治的97例EC患者，实时荧光定量聚合酶链式反应检测癌组织与癌旁组织中miR-25-3p、mRNA PTEN表达。通过TargetScan数据库预测miR-25-3p与PTEN关系，采用Pearson相关性分析EC组织中miR-25-3p与PTEN mRNA表达的相关性，并分析二者与EC病理参数的关系。结果:与癌旁组织比较，EC组织中miR-25-3p表达升高，PTEN mRNA表达降低（P<0.05）。TargetScan数据库预测发现，miR-25-3p与PTEN的3'非翻译区存在结合位点。Pearson相关性分析显示，EC组织中miR-25-3p与PTEN mRNA表达呈负相关（r=-0.721，P<0.001）。不同国际妇产科联盟（FIGO）分期、脉管侵犯、淋巴结转移EC组织中miR-25-3p、PTEN mRNA表达比较差异明显（P<0.05）。结论:EC组织中miR-25-3p高表达，PTEN mRNA低表达，与FIGO分期、脉管侵犯和淋巴结转移有关，其水平变化可为EC的临床诊疗提供参考。
Abstract
Objective:To investigate the expression of microRNA-25-3p (miR-25-3p) and phosphatase and tensin homolog (PTEN) in endometrial cancer (EC) and to analyze their relationship with EC pathological parameters.Methods:Ninety-seven EC patients admitted to Wuhan Maternal and Child Healthcare Hospital from January 2020 to November 2022 were selected, and their cancer and paraneoplastic tissues were used for real-time fluorescence quantitative polymerase chain reaction to detect miR-25-3p and mRNA PTEN expression. The TargetScan database was used to predict the relationship between miR-25-3p and PTEN. Pearson correlation was used to analyze the correlation between miR-25-3p and PTEN mRNA expression in EC tissues and to analyze their relationship with EC pathological parameters.Results:MiR-25-3p expression increased and PTEN mRNA expression decreased in EC tissues compared with paracancer tissues (P<0.05). Based on the TargetScan database, it was predicted that miR-25-3p has a binding site to the 3' untranslated region of PTEN. Pearson correlation analysis showed that miR-25-3p expression was negatively correlated with PTEN mRNA expression in EC tissues (r=-0.721, P<0.001). MiR-25-3p and PTEN mRNA expression was significantly different in EC tissues with different federation international of gynecology and obstetrics (FIGO) stages, vascular invasion, and lymph node metastasis (P<0.05).Conclusion:High expression of miR-25-3p and low expression of PTEN mRNA in EC tissues are associated with FIGO stage, vascular invasion, and lymph node metastasis, and the changes in their levels may provide a reference for the clinical management of EC.
基金项目
广西壮族自治区卫生健康委员会自筹经费科研课题(Z20201345)
万方数据