Relationship between miR-25-3p and PTEN expression and pathological parameters in endometrial cancer
Objective:To investigate the expression of microRNA-25-3p (miR-25-3p) and phosphatase and tensin homolog (PTEN) in endometrial cancer (EC) and to analyze their relationship with EC pathological parameters.Methods:Ninety-seven EC patients admitted to Wuhan Maternal and Child Healthcare Hospital from January 2020 to November 2022 were selected, and their cancer and paraneoplastic tissues were used for real-time fluorescence quantitative polymerase chain reaction to detect miR-25-3p and mRNA PTEN expression. The TargetScan database was used to predict the relationship between miR-25-3p and PTEN. Pearson correlation was used to analyze the correlation between miR-25-3p and PTEN mRNA expression in EC tissues and to analyze their relationship with EC pathological parameters.Results:MiR-25-3p expression increased and PTEN mRNA expression decreased in EC tissues compared with paracancer tissues (P<0.05). Based on the TargetScan database, it was predicted that miR-25-3p has a binding site to the 3' untranslated region of PTEN. Pearson correlation analysis showed that miR-25-3p expression was negatively correlated with PTEN mRNA expression in EC tissues (r=-0.721, P<0.001). MiR-25-3p and PTEN mRNA expression was significantly different in EC tissues with different federation international of gynecology and obstetrics (FIGO) stages, vascular invasion, and lymph node metastasis (P<0.05).Conclusion:High expression of miR-25-3p and low expression of PTEN mRNA in EC tissues are associated with FIGO stage, vascular invasion, and lymph node metastasis, and the changes in their levels may provide a reference for the clinical management of EC.
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