Association of IL-10 gene rs1554286 (G/A) polymorphism with prognosis of patients with primary hepatocellular carcinoma
Objective:To explore the association between the interleukin-10 (IL-10) gene single nucleotide polymorphism (SNP) rs1554286 (G/A) and the prognosis of patients with primary hepatic carcinoma (PHC) in Yanbian area, in order to provide theoretical basis for finding biomarkers that can predict the prognosis of PHC patients.Methods:From November 2003 to November 2015, a total of 191 patients with PHC were selected from Yanbian Tumor Hospital and Yanbian University Affiliated Hospital. DNA in blood samples was extracted using a whole blood DNA extraction kit. Polymerase chain reaction (PCR) primers were designed to amplify the DNA sequence containing the SNP site. Massarray SNP genotyping technology was used to analyze the genotype of the SNP site.Results:The IL-10 gene locus rs1554286 has G and A alleles and AA, GG, and GA genotypes. The genotype distribution frequency in the 191 patients with PHC was consistent with the law of Hardy-Weinberg equilibrium (P>0.05). There are 154 cases in the death group and 37 cases in the surviving group. There was a statistically significant difference in history of hepatitis and undergoing surgery or not between the two groups (P<0.05), but there was no significant difference in age, sex, ethnicity, drinking, smoking, or tumor stage between them (P>0.05). PHC patients with a history of hepatitis, surgery, and low tumor stage had a longer median survival time (P<0.05). The dominant model showed that the median survival time of PHC patients with rs1554286 AA genotype was longer than that of patients with GG+GA genotype (P<0.05).Conclusion:The IL-10 SNP rs1554286 (G/A) is associated with the prognosis of PHC patients. Having the rs1554286 A allele is a protective factor for the prognosis of PHC patients.
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