工作场所空气中活性药物成分现场采集和实验室检测方法的建立
Establishment of collection methods and laboratory testing methods for active pharmaceutical ingredient in the workplace air
方晓炜 1赵忠林 1张晶 1孙原 1王翔1
作者信息
- 1. 上海市化工职业病防治院,上海 200041
- 折叠
摘要
目的 通过建立工作场所空气中活性药物成分的现场采集和实验室检测方法,实现对常见9种典型活性药物成分的定性、定量分析.方法 于2021年12月,配制9种分析物混合液,后制成气溶胶状态进行模拟采样.选择玻璃纤维滤膜作为采样介质,以2.00 L/min的速率对模拟现场样进行15min采气收集,并将得到的滤膜样品于25％乙腈/75％甲醇洗脱液中洗脱,以液相色谱-三重四级杆质谱联用仪对洗脱液进行定性、定量分析.结果 本方法可有效采集空气中的活性药物成分,平均采样效率≥98.5％,且各项检测参数良好,线性相关系数r均＞0.9990;各目标分析物最低定量浓度范围为4.00x10-5～3.33×10-2mg/m3;平均加标回收率范围为97.6％～102.5％.结论 本方法可同时对空气中9种活性药物成分进行采集,并能够在后续实验室检测中进行准确定性和定量,符合国家测定方法研制标准.
Abstract
Objective To establish collection methods and laboratory testing methods for qualitative and quantitative analysis of 9 typical active pharmaceutical ingredient in the workplace air.Methods In December 2021,a mixed solution of nine analytes was prepared and then dispersed in aerosol state to simulate sampling.Glass fiber filter membrane was selected as air collector and collected active pharmaceutical ingredient in the air at a rate of 2.0 L/min for 15 minutes.Then,the obtained filter membrane samples were eluted with 25％ACN/75％MeOH.Finally,the eluent was qualitatively and quantitatively analyzed with liquid chromatography-triple quadrupole mass spectrometer.Results This method could effectively collect active pharmaceutical ingredient in the air,with an average sampling efficiency of more than 98.5％.The linear correlation coefficient r was greater than 0.9990.The lower limit of quantification for each analyte ranged from 0.6～500.0 ng/ml,and the average recovery rate ranged from 97.6％～102.5％.Conclusion This method could simultaneously collect 9 active pharmaceutical ingredient in the workplace air,and could provide accurate qualitative and quantitative analysis in subsequent laboratory tests.
关键词
工作场所空气/活性药物成分/采集/检测/液相色谱-三重四极杆质谱Key words
Workplace air/Active pharmaceutical ingredient/Collection/Liquid chromatography-triple quadrupole mass spectrometer引用本文复制引用
基金项目
上海市公共卫生体系建设三年行动计划(第五轮)(2020-2022)()
环境与职业卫生学重点学科建设项目(GWV-10.1-XK12)
出版年
2024
NETL
NSTL
万方数据