Objective To investigate the differences in terms of blood cerebrospinal fluid barrier immune surveillance injury by lead acetate and nano-lead sulfide exposure in order to provide basis for the study of their mechanism of nerve injury caused by exposure to lead and nano lead.Methods In June 2015,forty-five SPF SD male rats were randomly divided into control group,lead acetate group(20 mg/kg)and nano-lead sulfide group(20 mg/kg),with 15 rats in each group.The rats were intragastric five times a week,for nine weeks.The numbers of CD4+T lymphocytes in blood and cerebrospinal fluid were detected by flow cytometry.The levels of interleukin-4(IL-4)and interferon-γ(IFN-γ)in serum and cerebrospinal fluid were detected by ELISA.The expressions and distribution of intercellular cell adhesion molecule-1(ICAM-1)and CD4+T lymphocytes in choroid plexus were detected by laser confocal fluorescence immunoassay.The mRNA expression levels of IL-4,IFN-γ and ICAM-1 in the choroid plexus were detected by real-time PCR.Results Compared with the control group,the proportion of CD4+T lymphocytes in blood of rats in lead acetate group was increased,the proportions of CD4+T lymphocytes in cerebrospinal fluid of rats in lead acetate group and nano-lead sulfide group were increased,the contents of IL-4 and IFN-γ in serum of rats in lead acetate group and nano-lead sulfide group were increased,the content of IL-4 in cerebrospinal fluid of rats in lead acetate group and the contents of IL-4 and IFN-γ in cerebrospinal fluid of rats in nano-lead sulfide group were increased,the differences were statistically significant(P＜0.05).The fluorescence intensity of ICAM-1 and CD4+T lymphocytes in choriochoroid plexus of rats in lead acetate group and nano-lead sulfide group were stronger than those in control group,and the fluorescence intensity of CD4+T lymphocytes of rats in nano-lead sulfide group was weaker than that in lead acetate group.Compared with the control group,the mRNA expression levels of ICAM-1,IL-4 and IFN-γ in choriochoroids plexus of rats in lead acetate group and nano-lead sulfide group were increased,and the mRNA expression levels of ICAM-1 and IL-4 in nano-lead sulfide group were higher than those in lead acetate group,while the mRNA expression level of IFN-γ in nano-lead sulfide group was lower than that in lead acetate group(P＜0.05).Conclusion Exposure to lead and nano-lead sulfide can cause the increase of CD4+T lymphocytes,IL-4,IFN-γ and ICAM-1,which may be related to the damage to the immune surveillance of the blood cerebrospinal fluid barrier.And there is a difference in the injury caused by lead and nano-lead sulfide exposure.
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