摘要
目的 建立电感耦合等离子体质谱(ICP-MS)法同时测定全血中铍、钒、铬、锰、铁、钙、镁、钡、钴、镉、铜、锌、砷、硒、钛、锶、镍、钼、锡、锑、铊和铅22种元素含量的方法.方法 于2023年9月,通过优化仪器检测模式、样品前处理方式以及稀释倍数等参数确定分析条件,全血样品用体积分数为0.1％硝酸和0.05％曲拉通X-100混合溶液稀释后,用高速离心机以2 000 r/min离心2 min,取上清液进ICP-MS仪进行22种元素含量的测定,并对方法的检出限、精密度等指标进行分析.结果 22种元素在各自测定范围内均有良好的线性关系(r=0.999 1-0.999 9),检出限为0.003 μg/L～0.012 mg/L,批内精密度为0.5％～7.2％,批间精密度为0.4％～9.4％,平均回收率为80.6％～114.9％.结论 ICP-MS法测定全血中22种元素的效果良好,方法操作快速、简单,可用于临床全血中多元素的检测.
Abstract
Objective To establish a method for the simultaneous determination of 22 elements,including beryllium,vanadium,chromium,manganese,iron,calcium,magnesium,barium,cobalt,cadmium,copper,zinc,arsenic,selenium,titanium,strontium,nickel,molybdenum,tin,antimony,thallium and lead,in whole blood by inductively coupled plasma mass spectrometry(ICP-MS).Methods In September 2023,the analysis conditions were determined by optimizing the detection mode of the instrument,the pretreatment mode and the dilution factor of the samples,etc.Whole blood samples were diluted with a mixture of 0.1％nitric acid and 0.05％triton X-100,and centrifuged at 2000 r/min by high-speed centrifuge for 2 min.The supernatant was taken into inductively coupled plasma mass spectrometer to determine the content of 22 elements,and the detection limit and precision of the method were analyzed.Results The 22 elements had a good linear relationship in their respective measurement ranges(r=0.9991-0.9999),the detection limit ranged from 0.003 μg/L to 0.012 mg/L.The intra-batch precision ranged from 0.5％to 7.2％,the inter-batch precision ranged from 0.4％to 9.4％,and the average recoveries ranged from 80.6％to 114.9％.Conclusion ICP-MS method has a good effect on the determination of 22 elements in whole blood.The method is fast and simple,and can be used for clinical detection of multiple elements in whole blood.
基金项目
广西壮族自治区卫生健康委员会科技研究计划课题(Z-A20230411)
NETL
NSTL
万方数据