NLRP3炎症小体在姜黄素减轻七氟烷麻醉大鼠术后认知功能障碍中的作用
Role of NLRP3 inflammasomes in curcumin-induced reduction of sevoflurane-induced postoperative cognitive dysfunction in rats
张江玲 1杨亚利 1杜献慧 1张继兵 1王广治1
作者信息
- 1. 河南省人民医院麻醉与围术期医学科 郑州大学人民医院,郑州 450000
- 折叠
摘要
目的 评价NOD样受体蛋白3(NLRP3)炎症小体在姜黄素减轻七氟烷麻醉大鼠术后认知功能障碍中的作用.方法 SPF级健康雄性SD大鼠40只,17～18月龄,体质量580～600 g,采用随机数字表法分为4组(n=10):对照组(C组)、术后认知功能障碍组(P组)、姜黄素组(CU组)和姜黄素+NLRP3炎症小体激活剂组(CN组).采用吸入1.5％七氟烷探查腹腔的方法制备大鼠术后认知功能障碍模型.CU组和CN组大鼠姜黄素混悬液300 mg/kg灌胃,CN组大鼠尼日菌素钠盐5 mg/kg同时灌胃,1次/d,连续6d,C组和P组大鼠等量生理盐水灌胃.随后采用水迷宫实验测定大鼠穿越原平台位置次数和目标象限停留时间,HE染色法观察海马组织病理学结果,TUNEL染色法检测神经元凋亡情况,Western blot法检测NLRP3、Bcl-2和Bax表达.结果 与C组比较,P组大鼠穿越原平台位置次数减少,目标象限停留时间缩短,神经元凋亡率升高,NLRP3和Bax表达上调,Bcl-2表达下调(P＜0.05);与P组比较,CU组大鼠穿越原平台位置次数增加,目标象限停留时间延长,神经元凋亡率降低,NLRP3和Bax表达下调,Bcl-2表达上调(P＜0.05);与CU组比较,CN组大鼠穿越原平台位置次数减少,目标象限停留时间缩短,神经元凋亡率升高,NLRP3和Bax表达上调,Bcl-2表达下调(P＜0.05).结论 NLRP3炎症小体参与了姜黄素减轻七氟烷麻醉大鼠术后认知功能障碍的过程.
Abstract
Objective To evaluate the role of NOD-like receptor protein 3(NLRP3)inflamma-somes in curcumin-induced reduction of sevoflurane-induced postoperative cognitive dysfunction in rats.Methods Forty SPF healthy male Sprague-Dawley rats,aged 17-18 months,with body mass index of 580-600 g,were divided into 4 groups(n=10 each)by a random number table method:control group(C group),postoperative cognitive dysfunction group(P group),curcumin group(CU group),and curcumin+NLRP3 inflammasome activator group(CN group).The rat model of postoperative cognitive dysfunction was prepared by inhaling 1.5％sevoflurane to explore the abdominal cavity.Curcumin suspension 300 mg/kg was given by intragastric administration in CU group and CN group,and the rats received intragastric administra-tion of nidrisin sodium 5 mg/kg simultaneously in CN group,once a day for 6 consecutive days.Rats re-ceived the equal volume of normal saline instead in C group and P group.The frequency of crossing the orig-inal platform and time spent in the target quadrant were measured by the Morris water maze test.The his-topathological changes of hippocampus were observed by HE staining,neuronal apoptosis was detected by TUNEL staining,and the expression of NLRP3,Bcl-2 and Bax was detected by Western blot.Results Compared with C group,the frequency of crossing the original platform was significantly reduced,the time spent in the target quadrant was shortened,the apoptosis rate of neurons was increased,and the expression of NLRP3 and Bax was up-regulated,and the expression of Bcl-2 was down-regulated in P group(P＜0.05).Compared with P group,the frequency of crossing the original platform was significantly increased,the time spent in the target quadrant was prolonged,the apoptosis rate of neurons was decreased,and the expression of NLRP3 and Bax was down-regulated,and the expression of Bcl-2 was up-regulated in CU group(P＜0.05).Compared with CU group,the frequency of crossing the original platform was significantly reduced,the time spent in the target quadrant was shortened,the apoptosis rate of neurons was increased,and the ex-pression of NLRP3 and Bax was up-regulated,and the expression of Bcl-2 was down-regulated in CN group(P＜0.05).Conclusions The NLRP3 inflammasome is involved in curcumin-induced reduction of postop-erative cognitive dysfunction in sevoflurane-anesthetized rats.
关键词
NLR家族,热蛋白结构域包含蛋白3/姜黄素/七氟醚/认知障碍Key words
NLR family,pyrin domain-containing 3 protein/Curcumin/Sevoflurane/Cogni-tion disorders引用本文复制引用
出版年
2024
NETL
NSTL
万方数据