新型红细胞保存液对2型糖尿病大鼠贮存红细胞质量的影响
Effect of novel erythrocyte preservation solution on quality of erythrocytes in stored blood of T2DM rats
吴曼迪 1姚娜 1白玉 1崔应辉 1解小艺 1池永斌 2郭建荣1
作者信息
- 1. 海军军医大学附属公利医院麻醉与围术期医学部,上海 200315
- 2. 上海市炎症与慢病管理人工智能重点实验室,上海 200135
- 折叠
摘要
目的 评价新型红细胞保存液对2型糖尿病(T2DM)大鼠贮存红细胞质量的影响.方法 选择SPF级雄性SD大鼠40只,8周龄,体质量180～220 g,随机选择10只大鼠作为常规红细胞保存液组(A组);剩余30只大鼠制备T2DM模型,取造模成功的大鼠20只,采用随机数字表法分为2组(n=10):T2DM常规红细胞保存液组(C组)和T2DM新型红细胞保存液组(Y组).配制红细胞保存液,模拟术前贮存式自体输血流程,尾静脉取血分离红细胞后加入对应保存液.分别于采血即刻(T0)和贮存7d(T1)、14d(T2)、21d(T3)时,光镜下观察红细胞形态结构.分别于T0-T3和贮存28 d(T4)时,采用ELISA法检测红细胞2,3-二磷酸甘油酸(2,3-DPG)和ROS浓度.将贮存28 d的红细胞用PKH26标记后回输到大鼠体内,分别于回输后1、7、13和18 h时采用流式细胞术检测红细胞存活率.结果 光镜下,与A组相比,贮存不同时点C组红细胞损伤加重;与C组相比,贮存不同时点Y组红细胞损伤减轻.与A组比较,C组红细胞T0、T2-T4时2,3-DPG浓度降低,T0-T4时ROS浓度升高,回输后1、7和18 h时红细胞存活率降低(P＜0.05);与C组比较,Y组红细胞T0-T4时2,3-DPG浓度升高,T1、T3和T4时ROS含量降低,回输后1、7和18 h时红细胞存活率升高(P＜0.05).结论 新型红细胞保存液可改善T2DM大鼠贮存红细胞质量,提高回输后红细胞体内存活率.
Abstract
Objective To evaluate the effect of novel erythrocyte preservation solution on the quali-ty of erythrocytes in stored blood of type 2 diabetes mellitus(T2DM)rats.Methods Forty SPF male Spra-gue-Dawley rats,aged 8 weeks,weighing 180-220 g,were used in this study.Ten rats randomly selected served as conventional erythrocyte preservation solution group(group A).T2DM model was prepared in the remaining 30 rats.Twenty T2DM rats were divided into 2 groups(n=10 each)using a random number table method:T2DM conventional erythrocyte preservation solution control group(group C)and T2DM novel erythrocyte preservation solution observation group(group Y).Erythrocyte preservation solution was pre-pared to simulate the preoperative autologous blood donation process,and blood was collected from the tail vein to isolate the red blood cells,and then the corresponding preservation solution was added.Immediately after blood collection(T0)and at 7,14 and 21 days of preservation(T1-3),the morphological structure of erythrocytes was observed with a light microscope,and the concentrations of 2,3-diphosphoglycerate(2,3-DPG)and reactive oxygen species(ROS)were also determined by enzyme-linked immunosorbent assay at T0-T3 and 28 days of preservation(T4).PKH26 was used to label the erythrocytes stored in vitro for 28 days,and then the erythrocytes were transfused back into rats.The survival rate of erythrocytes was detected by flow cytometry at 1,7,13 and 18 h after retransfusion.Results The erythrocyte damage was aggravated at different time points of preservation in group C when compared with group A.Compared with group C,the damage to erythrocytes was significantly alleviated at different time points of preservation in group Y.Com-pared with group A,the concentration of 2,3-DPG in erythrocytes was significantly decreased at T0 and T2-T4,the concentration of ROS was increased at T0-T4,and the survival rate of erythrocytes was decreased at 1,7 and 18 h after retransfusion in group C(P＜0.05).Compared with group C,the concentration of 2,3-DPG in erythrocytes was significantly increased at T0-T4,the content of ROS was decreased at T1,T3 and T4,and the survival rate of erythrocytes was increased at 1,7 and 18 h after retransfusion in group Y(P＜0.05).Conclusions The novel erythrocyte preservation solution can improve the quality of stored erythro-cytes and increase the survival rate of erythrocytes in vivo after retransfusion in T2DM rats.
关键词
血液保存/红细胞/糖尿病,2型Key words
Blood preservation/Erythrocytes/Diabetes mellitus,type 2引用本文复制引用
基金项目
上海市浦东新区卫生系统新兴交叉学科建设项目(PWXx2020-05)
上海市浦东新区卫生系统重点学科群建设项目(PWZxq2022-05)
出版年
2024
NETL
NSTL
维普
万方数据