七氟烷对肺动脉高压大鼠右心室重构时心肌细胞Ca2+转运蛋白表达的影响
Effect of sevotlurane on Ca2+ transporter expression in cardiomyocytes during right ventricular re-modeling in rats with pulmonary arterial hypertension
翟文倩 1边燕飞 2宋悦孜 3李云飞 3许红 3郭志刚 4韩建阁1
作者信息
- 1. 天津大学胸科医院麻醉科,天津 300222;天津市心血管急危重症重点实验室,天津 300222
- 2. 包钢集团第三职工医院麻醉科,包头 014017
- 3. 天津市心血管急危重症重点实验室,天津 300222
- 4. 天津市心血管急危重症重点实验室,天津 300222;天津大学胸科医院心血管外科,天津 300222
- 折叠
摘要
目的 评价七氟烷对肺动脉高压大鼠右心室重构时心肌细胞Ca2+转运蛋白表达的影响.方法 清洁级健康成年雄性SD大鼠24只,8～10周龄,体质量200～250 g,采用随机数字表法分为4组(n=6):对照组(CM组)、七氟烷组(CS组)、野百合碱组(M组)和七氟烷+野百合碱组(S组).M组和S组大鼠腹腔注射野百合碱60 mg/kg,CM组大鼠腹腔注射野百合碱溶解剂.注射野百合碱后第1天时S组和CS组大鼠吸入2.5％七氟烷1 h,每周2次,每次间隔3 d.野百合碱注射后6周应用小动物经胸超声心动图法测定肺动脉加速时间和肺动脉射血时间.在3％七氟烷麻醉下暴露小鼠胸腔,灌注心脏,留取肺动脉分支血管和右心室心肌组织.HE染色观察肺小动脉管壁厚度和右心室心肌细胞横截面积,Western blot法检测右心室心肌细胞Ca2+转运蛋白的表达.结果 与CM组相比,M组大鼠肺动脉加速时间和肺动脉射血时间比值减小,右心室心肌细胞横截面积增大,肺小动脉管壁厚度增加,1型钠钙交换体和三磷酸肌醇受体表达上调,L-型电压依赖钙离子通道α1C亚基、兰尼碱受体、肌浆网钙泵2α和亲联蛋白-2表达下调(P＜0.01);与M组相比,S组大鼠肺动脉加速时间和肺动脉射血时间比值升高,右心室心肌细胞横截面积减小,肺小动脉管壁厚度减少,1型钠钙交换体和三磷酸肌醇受体表达下调,L-型电压依赖钙离子通道α1C亚基、兰尼碱受体、肌浆网钙泵2α和亲联蛋白-2表达上调(P＜0.01).结论 七氟烷改善肺动脉高压大鼠右心室重构的机制与调节心肌细胞Ca2+转运蛋白表达有关.
Abstract
Objective To evaluate the effect of sevoflurane on Ca2+ transporter expression in car-diomyocytes during right ventricular remodeling in rats with pulmonary arterial hypertension.Methods Twenty-four clean-grade healthy male Sprague-Dawley rats,aged 8-10 weeks,weighing 200-250 g,were divided into 4 groups(n=6 each)by the random number table method:control group(CM group),sevoflurane group(CS group),monocrotaline group(M group)and sevoflurane+monocrotaline group(S group).Monocrotaline 60 mg/kg was intraperitoneally injected in group M and group S,and monocrotaline lysate was intraperitoneally injected in group CM.The rats in S and CS groups inhaled 2.5％sevoflurane for 1 h,twice a week,at an interval of 3 days starting from the first day after injection of monocrotaline.Pulmo-nary artery acceleration time and pulmonary artery ejection time were measured by transthoracic echocardio-graphy at 6 weeks after monocrotaline injection.The chest was exposed under 3％sevoflurane anesthesia,the heart was perfused,and the pulmonary artery branch and right ventricular myocardial tissues were re-tained.The wall thickness of pulmonary arterioles and cross-section area of right ventricular cardiomyocytes were observed by HE staining.The expression of Ca2+transporter in right ventricular cardiomyocytes was de-tected by Western blot.Results Compared with CM group,the ratio of pulmonary artery acceleration time to pulmonary artery ejection time was significantly decreased,the cross-section area of right ventricular car-diomyocytes was increased,the wall thickness of pulmonary arteriole was increased,the expression of type 1 sodium-calcium exchange and inositol triphosphate receptor was up-regulated,and the expression of voltage-dependent L-type calcium channel α1C subunit,type 2 ryanodine receptor,sarcoplasmic reticulum calci-um pump 2α and proteinphilin-2 was down-regulated in M group(P＜0.01).Compared with group M,the ratio of pulmonary artery acceleration time to pulmonary artery ejection time was significantly increased,the cross-section area of right ventricular cardiomyocytes was decreased,the wall thickness of pulmonary arteriole was decreased,the expression of type 1 sodium-calcium exchange and inositol triphosphate recep-tor was down-regulated,and the expression of voltage-dependent L-type calcium channel α1C subunit,type 2 ryanodine receptor,sarcoplasmic reticulum calcium pump 2α and proteinphilin-2 was up-regulated in group S(P＜0.01).Conclusions The mechanism by which sevoflurane improves right ventricular re-modeling is related to regulating the expression of Ca2+transporter in cardiomyocytes of rats with pulmonary arterial hypertension.
关键词
七氟醚/肺动脉高压/心室重构/阳离子转运蛋白质类Key words
Sevoflurane/Pulmonary arterial hypertension/Ventricular remodeling/Cation transport proteins引用本文复制引用
基金项目
天津市科技计划(20JCZDJC00810)
恩泽疼痛管理医学研究项目()
天津市卫生健康科技项目(TJWJ2023MS027)
Enze Pain Management Medical Research Project()
出版年
2024
NETL
NSTL
万方数据