Objective To evaluate the relationship between microRNA-29a(miR-29a)and p53 up-regulated modulator of apoptosis(PUMA)in propofol-induced reduction of glucose deprivation(GD)-induced injury to human glioma cells.Methods Human glioma U87 cells were cultured in vitro to the loga-rithmic growth phase.Cells were then divided into 6 groups(n=24 each)by the random number table method:control group(group C),group GD,propofol+GD group(group P+GD),miR-29a inhibitor group(group I),miR-29a inhibitor+GD group(group I+GD)and miR-29a inhibitor+propofol+GD group(group I+P+GD).Cells were cultured in normal condition in group C.The culture medium was changed to glucose-free DMEM solution,and the cells were cultured for 12 h in group GD.In group P+GD,cells were incubated with propofol 10 μmol/L for 12 h and then incubated in glucose-free DMEM solution for 12 h.In group I,group I+GD and group I+P+GD,miR-29a inhibitor was transfected into cells using lipofectamine transfection kit,and then the cells were cultured for 48 h,and the other treatments were similar to those previously described in group P+GD.The cell survival rate,mitochondrial membrane potential and level of reactive oxygen species(ROS)were determined.The expression of miR-29a was detected by quantitative re-al-time polymerase chain reaction,and the expression PUMA was detected by Western blot.Results Com-pared with group C,the cell survival rate and mitochondrial membrane potential were significantly de-creased,the level of ROS was increased,the expression of miR-29a was down-regulated,and the expression of PUMA was up-regulated in group GD and group I(P＜0.05).Compared with group GD,the cell survival rate and mitochondrial membrane potential were significantly increased,the level of ROS was decreased,the expression of miR-29a was up-regulated,and the expression of PUMA was down-regulated in group P+GD,and the cell survival rate and mitochondrial membrane potential were significantly decreased,the level of ROS was increased,the expression of miR-29a was down-regulated,and the expression of PUMA was up-regulated in group I+GD(P＜0.05).Compared with group P+GD,the cell survival rate and mitochondrial membrane potential were significantly decreased,the level of ROS was increased,the expression of miR-29a was down-regulated,and the expression of PUMA was up-regulated in group I+P+GD(P＜0.05).Conclu-sions The mechanism by which propofol reduces glucose deprivation-induced injury to human glioma cells is related to up-regulation of miR-29a expression and down-regulation of PUMA expression.
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