Effects of Complex Enzymes on Nutrients Digestibility and Rumen Fermentation of Total Mixed Pellet Ration in vitro and the Growth Performance of Finishing Lamb
The two-step in vitro study was adopted to determine the nutrients digestibility and rumen fermentation parameters of total mixed pellet ration for lamb.There were three groups in this study,i.e.control group,treatment Ⅰ and treatment Ⅱ,which was a total mixed pellet ration with no enzyme,complex enzymes Ⅰ(100g/T)and complex enzymes Ⅱ(100g/T),respectively.One hundred and twenty lambs with an average weight of 20.83 kg and average age of 54 days were randomly divided into three groups to be fed with a total mixed pellet ration with no enzyme,complex enzymes Ⅰ(100g/T)and complex enzymes Ⅱ(100g/T),respectively.The total feeding period was 90 days,including a 7-d pre-feeding period and 83-d experiment period.The in vitro results showed that,compared with control,the digestibility of dry matter,neutral detergent fiber,and acid detergent fiber was significantly increased(P＜0.01)by 8.72％,11.52％and 19.62％,respectively,with the addition of complex enzymes Ⅱ.But the digestibility of crude protein was not affected(P＞0.05).No difference was detected in nutrients digestibility between the total mixed pellet ration of control and of treatment Ⅰ(P＞0.05).There was no difference in rumen pH among the three groups(P＞0.05).However,the concentration of ammonia nitrogen was significantly lower(P＜0.01)for treatment Ⅰ and treatment Ⅱ than that of control.Compared with control,the production of total volatile fatty acids was higher(P＜0.01)for treatment Ⅱ.The feeding experiment showed that treatment Ⅱ significantly increased the dry matter intake,the average daily gain and the feed conversion ratio(P＜0.05)compared to control.It was concluded that the addition of complex enzymes could affect the nutrients digestibility and rumen fermentation in vitro.The feeding experiment showed that the addition of 100 g/t complex enzymes could improve the feed conversion ratio and the average daily gain.
Complex enzymeFinishing lambIn vitro digestionRumen fermentationAverage daily gain
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