Study on the Effect of Low-temperature(0°C)Preservation of Bovine Semen
In order to study the effect of a Tris-egg yolk diluent 0℃(ice water mixture)preservation of bovine semen,the test select-ed 5 heads of Simensthal cattle,the semen collected from each cattle was divided into equal volumes,and diluted with the test group diluent(Tris-egg yolk patent diluent)and the control group diluent(basic diluent)at a ratio of 1:3,and then preserved at 0℃(ice water mixture).The sperm viability,acrosome integrity and plasma membrane integrity were detected at 0,1,2,4,6,8,10,and 12 d after preservation,and artificial insemination was performed with the semen preserved for 5 d,and the conception rate was cal-culated.The results showed that:the sperm viability of group A was significantly greater than that of group B after preservation for 4～10 d(P<0.05);the sperm viability of group A was greater than 50%after preservation for 10 d;the acrosome integrity and plas-ma membrane integrity of bovine sperm preserved at 0℃gradually decreased with the prolongation of preservation time,but there was no significant difference between different treatments(P>0.05).The conception rate of bovine sperm preserved in group A was significantly higher than that of group B.It is clear that the preservation effect of bovine semen preserved at 0℃with Tris-egg yolk diluent is better,and the conception rate is higher.
万方数据
维普
