Association between MTPN Gene Polymorphism and Growth Traits in Sheep
To investigate the correlation between myotrophin(MTPN)gene polymorphisms and growth characteristics in sheep,molecular genetic markers linked to growth were scrutinized.A total of 1557 sheep were enlisted for this inquiry,comprising 536 specimens of Lanzhou large-tailed sheep,904 Tan sheep,and 117 Tibetan sheep.PCR-SSCP in conjunction with sequencing was employed to discern single nucleotide polymorphisms and genotypes,to delve into the potential correlation between these mutational sites and various growth traits.The findings revealed the presence of a variant locus,g.101185485G＞A,in Lanzhou large-tailed sheep,Tan sheep,and Tibetan sheep,with AA and AG genotypes observed at this locus.The g.101185485 G＞A locus exhibited low polymorphism(PIC＜0.25)and was in Hardy-Weinberg equilibrium(P＞0.05)across all three sheep populations.Among Lanzhou large-tailed sheep,Tan sheep,and Tibetan sheep,the AA genotype prevailed,exhibiting significantly greater body height and chest circumference in Lanzhou large-tailed sheep,body height and chest circumference in Tan sheep,and body height in Tibetan sheep with the AA genotype compared to those with AG genotype(P＜0.05).Thus,it is evident that the g.101185485 G＞A variant of the MTPN gene can serve as a valuable genetic marker locus in the molecular breeding of sheep and is favored in breeding endeavors.
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