In order to understand the prevalence of BVDV in a large-scale cattle farm in Jilin Province and formulate a purification plan,a BVDV ELISA kit was used to investigate the etiology of blood and fecal samples collected from a large-scale cattle farm in Jilin Province from March 2022 to October 2023.The results showed that the positive rates of BVDV antibody and antigen in the whole cattle farm were 2.72%and 0.85%,respectively.There were 5 positive antigens(including 1 adult cattle,1 reserve cattle,and 3 calves)and 16 positive antibodies(including 5 adult cattle,5 reserve cattle,and 6 calves).Through the direct elimination of BVDV positive cattle,and through three re-examinations,BVDV persistent infected cattle were screened and eliminated,and the negative cattle were immunized.After 1 year of monitoring and elimination,the BVDV pathogen detection rate of the scale cattle farm was 0,achieved good purify results.