牛病毒性腹泻净化效果研究
Study on Purification Effect of Bovine Viral Diarrhea
高佳滨 1李成侠 2赵旭东 2郭艳芹 1鲁英 1王润彬 2马秀兰2
作者信息
- 1. 白城市畜牧科学研究院,吉林白城 137000;吉林省肉牛高效养殖厅地共建科技创新中心,吉林白城 137000
- 2. 吉林省德信生物工程有限公司,吉林白城 137000;吉林省肉牛高效养殖厅地共建科技创新中心,吉林白城 137000
- 折叠
摘要
为了解吉林省某规模牛场BVDV的流行情况,并制定净化方案,应用BVDV ELISA试剂盒对 2022 年 3月—2023年10月采集吉林省某规模牛场的血液和粪便样本进行病原学调查.结果表明:该牛场全群BVDV抗体、抗原阳性率分别为 2.72%、0.85%,抗原阳性 5 头(其中成年牛 1 头,后备牛 1 头,犊牛 3 头),抗体阳性 16 头(其中成年牛 5 头,后备牛 5 头,犊牛 6 头).对检测为BVDV阳性的牛直接淘汰,并通过 3 次复检,筛选出BVDV持续性感染牛,对其进行淘汰,并对检测阴性牛免疫.经过长达 1年的监测及淘汰,该规模牛场BVDV病原学检出率为0,取得了良好净化效果.
Abstract
In order to understand the prevalence of BVDV in a large-scale cattle farm in Jilin Province and formulate a purification plan,a BVDV ELISA kit was used to investigate the etiology of blood and fecal samples collected from a large-scale cattle farm in Jilin Province from March 2022 to October 2023.The results showed that the positive rates of BVDV antibody and antigen in the whole cattle farm were 2.72%and 0.85%,respectively.There were 5 positive antigens(including 1 adult cattle,1 reserve cattle,and 3 calves)and 16 positive antibodies(including 5 adult cattle,5 reserve cattle,and 6 calves).Through the direct elimination of BVDV positive cattle,and through three re-examinations,BVDV persistent infected cattle were screened and eliminated,and the negative cattle were immunized.After 1 year of monitoring and elimination,the BVDV pathogen detection rate of the scale cattle farm was 0,achieved good purify results.
关键词
牛病毒性腹泻/净化/抗体检测/抗原检测Key words
Bovine viral diarrhea/Purification/Antibody detection/Antigen detection引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据