Exploring the Correlation of Apparent Indices of Boar Semen Before and After Freezing Based on Canonical Correlation Analysis
This study aimed to investigate the correlation between the apparent indicators of boar semen before and after freezing using canonical correlation analysis,to understand the impact of the freezing process on semen quality.Semen from 30 healthy boars aged 1 to 2 years(15 French Landrace and 15 Large White)was frozen 2 to 4 times.The pre-freeze indicators,including tion motility,viability,and density,as well as post-freeze indicators,such as motility,corrected motility,and the average motility at 10 minutes and 2 hours after thawing,were analyzed.The results showed that the coefficient of variation for semen density before freezing was 42.14％,indicating comprehensive coverage of the samples.The average activity at 10 min after thawing was significantly positively correlated with the pre-dilution activity(P＜0.01).The average viability at 10 min after thawing was significantly positively correlated with pre-dilution viability(P＜0.05)and extremely significantly positively correlated with pre-dilution viability(P＜0.01).The average corrected viability at 2 h after thawing was significantly positively correlated with the pre-dilution viability(P＜0.05).The first and second pairs of canonical correlation coefficients were 0.639 and 0.580,respectively.The shared variance of the first pair of canonical correlations reached 0.408,indicating that the quality of raw semen could contribute up to 40.8％to the quality of frozen semen.Through canonical correlation analysis,the correlation between pre-freeze and post-freeze semen quality indicators improved from a maximum simple correlation of 0.538 to 0.639 between the comprehensive indicators U1 and V1,indicating that canonical correlation analysis can better utilize pre-freeze semen indicators to predict post-freeze semen quality.
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