Determination of naringin in Qiyelin granules by high performance liquid chromatography
Objective To establish a high performance liquid chromatography method for determination naringin in Qiyelin granules.Methods Kromasil C18 column(250.0 mm × 4.6 mm,5 μm) was used; the mobile phase was acetonitrile∶ 4％ acetic acid (17∶ 83).The detection wavelength was 224 nm; the flow rate was 1 ml/min and the column temperature was 30 °C.Results This method determinated the naringin effectively and avoided the impurity.The linear range of naringin was at 0.08-2 μg (r =0.9999),relative standard deviation (RSD) was 1.97 ％ (n =5).The average recovery of naringin was 102.6％,RSD =1.86％ (n =6).Conclusions The method of Qiyeling particles in naringin determined by high performance liquid chromatography is simple and repeatable.The result is stable.It can be used for determination the content of naringin in Qiyelin granules.
High performance liquid chromatographyQiyelin granulesNaringin
NETL
NSTL
万方数据
维普
