痰标本中金黄色葡萄球菌的分离及耐药性分析
Sputum specimens staphylococcus ball separation and resistance analysis
赵建平 1周秀岚2
作者信息
- 1. 010010呼和浩特,内蒙古自治区人民医院检验科
- 2. 内蒙古医科大学附属医院药学部
- 折叠
摘要
目的 回顾性分析痰标本中金黄色葡萄球菌的临床分布和耐药情况,为预防控制措施的制定提供依据.方法 对2009年1月至2013年9月内蒙古自治区人民医院临床分离的309株金黄色葡萄球菌进行分析,细菌鉴定和药敏试验采用VITEK 2 Compact全自动细菌培养鉴定仪.结果 耐甲氧西林金黄色葡萄球菌(MRSA)的分离率为78.6％(243/309),β内酰胺酶阳性金黄色葡萄球菌分离率为90.0％(278/309);金黄色葡萄球菌的病区来源主要为神经内科[30.7％ (95/309)]、重症监护病房[22.0％(68/309)]、神经外科[16.5％(51/309)]、呼吸内科[9.4％ (29/309)].金黄色葡萄球菌对万古霉素、替考拉宁、奎奴普丁/达福普汀和呋喃妥因的耐药率≤2.1％,MRSA与对甲氧西林敏感的金黄色葡萄球菌(MSSA)以及β内酰胺酶阳性金黄色葡萄球菌与β内酰胺酶阴性金黄色葡萄球菌间的耐药性差异无统计学意义(P＞0.05);对复方新诺明的耐药性MRSA低于MSSA(P ＜0.05),β内酰胺酶阳性低于β内酰胺酶阴性金黄色葡萄球菌(P＜0.05).对其他抗菌药物的耐药性MRSA高于MSSA(P ＜0.05);β内酰胺酶阳性高于β内酰胺酶阴性金黄色葡萄球菌,但除左旋氧氟沙星外(P＜0.05),其他差异均无统计学意义(P＞0.05).结论 MRSA和β内酰胺酶阳性金黄色葡萄球菌的检出率和耐药性较高,临床应依据药敏试验监测结果合理选择抗菌药物.
Abstract
Objective To analyze the clinical distribution and drug resistance of sputum specimens staphylococcus ball.Methods From January 2009 to September 2013,309 strains of clinical separation of staphylococcus aureus were analyzed.The bacteria identification and drug sensitive test were detected by VITEK 2 Compact automatic bacterial culture appraisal instrument.Results The separation rates ofmethicillin resistant staphylococcus aureus(MRSA) and beta-lactamase positive staphylococcus aureus were 78.6％ (243/309) and 90.0％ (278/309) ; the main sourceof staphylococcus aureus was neurology department,accounting for 30.7 ％ (95/309).The resistant rate of staphylococcus aureusto vancomycin,teicoplanin,quinupristin/dafoe leptin andnitrofurantoin was all ＜ 2.1％.There were no significant differences regardingbetween MRSA and methicillin sensitive staphylococcus aureus (MSSA),drug resistancebetween beta lactamase positive staphylococcus aureus and beta lactamase negative staphylococcus aureus (all P ＞ 0.05).Beta lactamase positive staphylococcus aureus was lower than beta lactamase negative staphylococcus aureus (P ＜ 0.05).The drug resistance of MRSA was higher than that of MSSA (P ＜ 0.05).Beta lactamase positive staphylococcus aureus was higher than beta lactamase negative staphylococcus aureus.Conclusion The detective rate and drug resistance of MRSA and beta lactamase positive staphylococcus aureus are high and the reasonable choice ofantibacterial drugs should be based on clinical drug sensitive test monitoring results.
关键词
金黄色葡萄球菌/耐甲氧西林金黄色葡萄球菌/耐药率/复方新诺明/β内酰胺酶Key words
Staphylococcus aureus/Methicillin resistant staphylococcus aureus/Resistant/Cotrimoxazole/Beta-lactamase引用本文复制引用
基金项目
内蒙古自治区卫生厅医疗卫生科研计划(2010102)
出版年
2014
NETL
NSTL
维普
万方数据