Relationship between serum purinergic receptor P2X7 and YTH structural domain N6-methyladenosine RNA binding protein 2 and hemorrhagic transformation after intravenous thrombolysis in patients with acute ischemic stroke
Objective To investigate the relationship between serum purinergic receptor P2X7(P2RX7)and YTH structural domain N6-methyladenosine RNA binding protein 2(YTHDF2)and hemorrhagic transformation(HT)after intravenous thrombolysis in patients with acute ischemic stroke(AIS).Methods A total of 192 AIS patients admitted to Shaanxi Kangfu Hospital from January 2020 to December 2023 were selected as the AIS group(after thrombolysis,it was divided into hemorrhagic transformation group and non-hemorrhagic transformation group),and 150 healthy people in the same period were selected as the control group.Serum P2RX7 and YTHDF2 levels were detected by enzyme-linked immunosorbent assay.With HT after intravenous thrombolysis as the dependent variable,a Logistic regression model was established to determine the related factors of HT in AIS patients after intravenous thrombolysis.The receiver operating characteristic curve was drawn to evaluate the predictive value of serum P2RX7 and YTHDF2 levels for HT.Results The serum levels of P2RX7 and YTHDF2 in AIS group were higher than those in control group[(1.6±0.3)μg/L vs(1.2±0.3)μg/L,(1.32±0.26)μg/L vs(1.03±0.16)μg/L](both P＜0.001).The levels of serum P2RX7 and YTHDF2 in the hemorrhagic transformation group were higher than those in the non-hemorrhagic transformation group(both P＜0.001).Multivariate Logistic regression analysis model results showed that high National Institutes of Health Stroke Scale score,cardiogenic embolism,high P2RX7 level and high YTHDF2 level were independent risk factors for HT in AIS patients after intravenous thrombolysis(all P＜0.05).The area under the curve of serum P2RX7 combined with YTHDF2 for predicting hemorrhagic transformation was 0.886,which was larger than 0.780 and 0.784 predicted by P2RX7 or YTHDF2 alone(both P＜0.001).Conclusion Elevated serum P2RX7 and YTHDF2 levels are independent risk factors for HT in patients with AIS after intravenous thrombolysis,and serum P2RX7 combined with YTHDF2 detection has a high predictive value for HT.
万方数据
维普
