Investigate of Pharmacokinetics of Karanjin in SD Rats by HPLC-MS/MS
Objective:To establish a quantitative analysis method for the determination of karanjin in rat plasma and study its pharmacokinetics.Methods:SD rats were intragastrically administered with karanjin(10 mg·kg-1).The plasma samples were pretreated by protein precipitation method and then detected quantitatively by HPLC-MS/MS.Schisandrin was employed as an internal standard.The separation was performed on an Agilent Poroshell 120 EC-C18(50 mm×3.0 mm,2.7 μm)column with acetonitrile(A)-0.1％methanoic acid solution(B)as mobile phase at a flow rate of 0.3 mL·min1.Electrospray ionization(ESI)source and positive ion multiple reaction monitoring(MRM)mode were used for scanning,and the results showed that karanjin[M+H]+m/z 293.1→277.0,schisandrin[M+H]+m/z 433.2→384.2;the pharmacokinetic parameters were calculated by DAS software.Results:In the range of 1.5625-1600 ng·mL-1,karanjin showed a good liner relationship in plasma(r=0.9997),with a low limit of quantification of 1.5625 ng·mL-1,precision(RSD)and accuracy(RE)were less than 20％.The precision RSD and accuracy RE of low medium and high quality control samples were less than 15％.The Tmax,t1/2,Cmax and AUC0→t of female SD rats were 3 h,1.95 h,1214.85 ng·mL-1 and 11920.43 ng·mL-1·h respectively,while that of male rats were 3 h,1.19 h,1221.57 ng·mL-1 and 9983.40 ng·mL-1·h respectively.Conclusion:The established method meets the basic requirements of quantitative analysis of biological samples,and can be used for the content determination and pharmacokinetic evaluation of karanjin in rat plasma.The bioavailability of karanjin in female SD rats is higher.
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万方数据
维普
