泻白散质量标志物的发现及其作用机制预测分析
Discovery of Q-markers of Xiebai Powder and Predictive Analysis of Its Mechanism of Action
马天宇 1肖智杰 1段卓然 1常梓睿 1牟景龙 1孟永海 1翟春梅1
作者信息
- 1. 黑龙江中医药大学教育部北药基础与应用研究重点实验室,哈尔滨 150040
- 折叠
摘要
目的:旨在明确泻白散质量标志物并预测其作用机制,确保临床疗效.方法:通过Q-markers"五原则"筛选泻白散质量标志物;采用TCMSP、Swiss Target Prediction等数据库获取Q-markers潜在作用靶点,构建质量标志物的蛋白互作网络.通过KEGG、GO、拓扑富集分析构建"关键靶点-关键通路-关键生物过程"网络,进而预测泻白散质量标志物的潜在作用机制.结果:研究显示,氧化白藜芦醇、绿原酸、阿魏酸、甘草素、甘草酸和芒柄花素6个化合物可作为泻白散的潜在质量标志物,这些物质可能通过调节炎症反应中的一氧化氮生物合成过程、AGEs-RAGE信号通路等途径发挥作用.结论:通过初步探讨泻白散的质量标志物及其作用机制,为泻白散质量控制与临床应用提供科学依据.
Abstract
Objective:To identify Q-markers and predict their mechanisms of action to ensure clinical efficacy.Methods:The quality markers for Xiebai Powder were screened by Q-markers"Five Principles".Databases such as TCMSP and Swiss Target Prediction were used to obtain potential targets of Q-markers,in order to construct a protein interaction network for Q-markers.Through KEGG,GO,and topological enrichment analysis,the"key target-key signal pathway-key biological process"network was constructed to predict the potential mechanism of the Q-markers of Xiebai Powder.Results:This study showed that oxyresveratrol,chlorogenic acid,ferulic acid,liquiritigenin,glycyrrhizic acid and formononetin could be used as potential quality markers of Xiebai Powder.These substances might play a role in regulating the nitric oxide biosynthesis process and AGEs-RAGE signaling pathway in inflammatory response.Conclusion:This article preliminarily explores the quality markers and mechanism of Xiebai Powder,aiming to provide scientific basis for the quality control and clinical application of Xiebai Powder.
关键词
泻白散/五原则/质量标志物/作用机制/网络药理学Key words
Xiebai Powder/five principles/Q-markers/action mechanism/network pharmacology引用本文复制引用
基金项目
哈尔滨市科技创新人才研究专项资金项目(2017RAQXJ125)
黑龙江中医药大学研究生创新科研项目(2022yjscx055)
黑龙江省博士后科研启动金资助项目(LBH-Q15137)
出版年
2024
NETL
NSTL
万方数据