Study on the Pseudo Virus-based Neutralization Assay for Rabies Virus Neutralization Antibody Test
Objective:To analyze the ability of pseudo virus-based neutralization assay(PBNA)to detect rabies virus neutralization antibody levels in different samples in different laboratories,and compare its correlation with classic rapid fluorescent focus inhibition test(RFFIT)and mouse neutralization test(MNT).Methods:A total of 9 laboratories were used to test rabies virus neutralization antibody in 6 samples by PBNA,RFFIT and MNT methods respectively,and 3 to 5 repeated experiments were carried out in each laboratory.Paired t test and Pearson correlation coefficient were used to analyze the results of the three detection methods.Results:The Pearson correlation coefficient between PBNA and RFFIT,PBNA and MNT,RFFIT and MNT were 0.985,0.988 and 0.974 separately.The geometric mean titer of antibody was tested by t test,and there was no significant difference(P＞0.05).Conclusion:The quantitative results of the repeated detection of 6 samples by three methods are highly correlated.PBNA method can be used as a substitute or supplementary method for RFFIT and MNT methods in Chinese Pharmacopoeia for the detection of rabies virus neutralization antibody in clinical serum of human rabies vaccine,rabies human immunoglobulin and anti-rabies serum.
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