Identification of Juniperus Folium the Mongolian Medicine and Its Adulterants Based on DNA Barcoding Technique
Objective:To identify the Juniperus Folium of Mongolian medicine and its adulterants by DNA barcode technology.Method:The DNA extraction and amplification of 11 samples of Juniperus Folium and the adulterants Sabina Folium were carried out by using the intemationally recognized barcode sequences ITS2,psbA-trnH,matK and rbcL.Codon Code Aligner was adopted to align the sequence,and MEGA software was used to analyze the mutation sites and Neighbor-Joining(NJ)cluster of the splice sequence,and its average intra-specific and inter-specific genetic distance were calculated.Results:The success rates of PCR amplification products of the four primers were ITS2 100％,psbA-trnH 100％,rbcL 100％and matK 0％,respectively.The ITS2 sequences and psbA-trnH sequences could distinguish the Juniperus Folium and its adulterants by comparison of variation sites.The results of NJ cluster analysis showed that the psbA-trnH sequences of Juniperus Folium and its adulterants could be clustered into one branch respectively,and the average interspecific genetic distance ofpsbA-trnH sequences was significantly greater than the average intraspecific genetic distance.Conclusion:The psbA-trnH sequences can effectively distinguish Juniperus Folium from Sabina Folium,and can be used as barcode sequence to identify Juniperus Folium and the adulterants Sabina Folium,which will provide support for the identification of Mongolian medicinal materials Juniperus Folium and the adulterants Sabina Folium.
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