Content Changes of 7 Components in Daphne genkwa Decoction Pieces Before and After Processing Determined by HPLC and UPLC-MS Method
Objective:To establish HPLC method and UPLC-MS method for the determination of tiliroside,luteolin,apigenin,chlorogenic acid,hydroxy genkwanin,genkwanin and yuanhuacine in Daphne genkwa.To analyze the contents change of 7 chemical components before and after processing.Methods:The contents of tiliroside,luteolin,apigenin,chlorogenic acid,hydroxy genkwanin and genkwanin were determined by HPLC.The chromatographic column was Agilent Eclipse Plus C18(250 mm × 4.6 mm,5 μm)with mobile phase gradient elution of acetonitrile(A)-0.1%formic acid(B).The flow rate was 1.0 mL·min-1 and the injection volume was 10 µL.The content of yuanhuacine was analyzed by UPLC-MS.The chromatography was performed on ACQUITY UPLC HSS T3 C18 column(2.1 mm × 100 mm,1.8 μm)with gradient elution of acetonitrile(A)-0.1%formic acid(B)as mobile phase at the flow rate of 0.3 mL·min1.The column temperature was 35 ℃ and the injection volume was 1 μL.Results:The contents of the 7 components in the order of high to low were genkwanin>chlorogenic acid>tiliroside>apigenin>hydroxy genkwanin>luteolin>yuanhuacine.The contents of 7 components in Daphne genkwa were changed to a certain extent after processing,and the content of chlorogenic acid was increased and decreased after processing,while the change was not obvious.The contents of tiliroside and apigenin were decreased after processing.The contents of luteolin,hydroxy genkwanin and genkwanin were increased after processing.For yuanhuacine,its content was tended to decrease after processing.Conclusion:The method is simple,sensitive and efficient,and provides technical support for investigating the quality changes of Daphne genkwa decoction slices before and after processing.
Daphne genkwaprocessingchemical componentscontent determinationliquid chromatographyliquid mass chromatography