Prediction and Analysis of Quality Markers of Codonopsis Radix by UPLC Fingerprint and Chemometric Analysis
Objective:To establish the UPLC fingerprints of Codonopsis Radix and analyze it combined with the chemometric method,to predict and analyze the quality markers(Q-Marker)of Codonopsis Radix in order to provide a scientific basis for the quality of Codonopsis Radix.Methods:HPLC was launched on a ACQUITY UPLC®BEH C18 column(2.1 mm×100 mm,1.7 μm)by gradient elution with a mobile phase of acetonitrile-0.1％phosphoric acid aqueous solution at a flow rate of 0.3 mL·min-1,detection wavelength of 267 nm,column temperature of 30 ℃,and an injection volume of 2 μL.Gradient elution was performed using Agilent ZORBAX SB-C18 column and acetonitrile-0.1％phosphoric acid aqueous solution by HPLC.The UPLC fingerprints of 21 batches of Codonopsis Radix were established and the similarity was evaluated.The main differential components were screened by chemometric methods such as cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA),and the Q-Marker of Codonopsis Radix was predicted.Results:The UPLC fingerprint of Codonopsis Radix was established,and 16 common peaks were confirmed,and 7 common peaks were identified by reference products,which were Adenosine,Tryptophan,Syringin,Chlorogenic Acid,Tangshenoside I,Lobetyolin and Atractylenolide Ⅲ.The similarity of fingerprints was greater than 0.85.Using HCA,PCA and OPLS-DA,Tangshenoside I,Lobetyolin,Adenosine and Tryptophan were identified as important differential components of Codonopsis Radix from different regions.These components could be used as potential Q-Marker of Codonopsis Radix.Conclusion:The potential Q-Marker of Codonopsis Radix is predicted by fingerprint combined with chemometrics analysis,which provided a reference for quality evaluation of Codonopsis Radix.
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