Study on the Quality Control of Dahuoluo Pills Based on Adulteration Detection of Red Ginseng
Objective:To establish a method for detecting red ginseng adulterated with American ginseng in Dahuoluo Pills to provide an important support for supervising its quality.Methods:Taking pseudo-ginsenoside F11,the characteristic component of American ginseng,as the adulteration detection index,an UPLC-MS/MS analysis was performed on C18 column(100 mm×2.1 mm,1.8 μm),using acetonitrile and 10 mmol·L-1 ammonium formate solution as mobile phase for gradient elution at a flow rate of 0.3 mL·min-1,and column temperature of 40 ℃.The analyte was detected by electrospray anion scanning and multiple reaction monitoring mode.Results:There was no obvious matrix effect.Pseudo-ginsenoside F11 showed a good linearity in the range of 0.0117-1.167 μg·mL-1(R2＞0.9990),and also has a linear relationship with different adulteration proportion.The average recovery was 95.2％with RSD of 1.73％(n=6).Among the 96 samples,pseudo-ginsenoside F11 was detected in 37 samples,and 5 samples from an enterprise exceeded the proposed detection limit,leading to the disqualified rate was 5.2％.Conclusion:This study comprehensively develops a method for detecting red ginseng adulterated with American ginseng in Dahuoluo Pills,and the established method is sensitive and accurate,which can be applied to quality inspection of Dahuoluo Pills.
Dahuoluo Pillred ginsengAmerican ginsengpseudo-ginsenoside F11quality control
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