New Enzyme-linked Immunosorbent Assay for Detection of Antibody to Varicella-zoster Virus
Objective:Varicella-zoster Virus(VZV)glycoproteins are the antigens that have been demonstrated to be a highly accurate indicator of VZV-specific immunity.This study established an enzyme-linked immunosorbent assay(ELISA)using recombinant glycoproteins as the coated antigens for evaluation of the serum antibody level after varicella vaccination.Methods:The ELISA coated antigen was screened,and then the recombinant proteins gE and gB were used as the coated antigens,and the W1044 international reference substance was used as the standard,and the ELISA method of VZV antibody was preliminarily established and verified.The established ELISA method was compared with the fluorescent antibody to membrane antigen(FAMA)method.Results:Using gE and gB as coated antigens,ELISA method was successfully established.Using linear fitting,the standard curve showed a good linear relationship within the antibody concentration range of 0.25-4 mIU·mL-1,R2＞0.990,with 85％-120％recovery rate,0.25 mIU·mL-1 limit of quantification,and less than 15％of precision CV.Compared with the FAMA method,the ELISA showed a sensitivity of 89％,specificity of 93％and overall conformity rate of 90％.Conclusion:This study successfully established a VZV antibody ELISA detection method,which can be used as a sensitive and specific quantitative method to evaluate clinical serum antibody level after varicella vaccination.
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