Quality Control of Aconiti Vilmoriniani Radix with Toxic Herbs of Ethnic Yi Herbal Medicine Yunnan Hongyao Capsules
Objective:To explore the quality control method of Aconiti Vilmoriniani Radix with toxic herbs of Yunnan Hongyao Capsules,and to provide a basis for the formulation of quality standards.Methods:Microscopic identification method was used to screen the specific microscopic features of the preparation.The limit test of yunaconitine in Aconiti Vilmoriniani Radix was determined by HPLC.The column was Eclipse Plus C18(4.6 mm×250 mm,5 μm),and the mobile phase was methanol-buffered salt solution(water-triethylamine-phosphoric acid was 600∶2∶1)(40∶60)at the flow rate of 1.0 mL·min-1.The detection wavelength was 260 nm and the column temperature was 30 ℃.Results:The stone cell characteristics of Aconiti Vilmoriniani Radix had a strong specificity.Determined by HPLC,the injection volume of Yunaconitine showed a good linear relationship in the range of 12.24-367.18 ng(r＞0.9998);the average recovery was 93.2％with RSD of 1.96％(n=6).The amount of yunaconitine in 15 batches of samples was lower than the proposed limit.Conclusion:The stone cell characteristics can be used to identify Aconiti Vilmoriniani Radix,and the separation effect of the limit test method of yunaconitine is good,which can provide a basis for the control of Aconiti Vilmoriniani Radix in the compound preparation of Yunnan Hongyao capsules.
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