Objective:To investigate the effects of proanthocyanidin A2 (PCA2) on the proliferation and migration of human prostate cancer cells (PC-3 cells) by cytological tests in vitro,and to elucidate its potential anti-cancer molecular mechanisms based on network pharmacology. Methods:The effects of varying concentrations of PCA2 on PC-3 cell proliferation were assessed using CCK8 assay. Colony formation and scratch assays were used to determine the effects of PCA2 on PC-3 cell proliferation and migration. Predicted targets of PCA2 and prostate cancer were identified through Similarity Ensemble Approach and other databases,and a protein-protein interaction network was constructed using String. GO and KEGG functional enrichment analyses were performed using the OmicShare Tools database to predict the mechanisms of action. Results and Conclusion:PCA2 significantly inhibited the proliferation and migration of PC-3 cells,and the inhibitory effect is positively correlated with the concentration of PCA2. Through network pharmacology,95 targets were identified between PCA2 and prostate cancer,with EGFR,ALB,HSP90AA1,and MMP9 being the core targets. GO and KEGG functional enrichment analyses indicated that its main mechanism related to the PI3K/AKT signaling pathway.
关键词
原花青素A2/前列腺癌/人前列腺癌细胞系/迁移增殖/PI3K/AKT信号通路
Key words
proanthocyanidin A2/prostate cancer/PC-3 cells/migration and proliferation/PI3K/AKT signaling pathway
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