Nuclease-dependent genome editing technology were capable of modifying DNA sequences at the genomic level,including genetic modifications such as the insertion,knockout,replacement and point mutation of specific DNA segments.The CRISPR/Cas9 gene editing technology developed in 2012,it had been garnered widespread attention and application with the characteristics of high efficiency,high throughput,high safety,easy operation and simple realization.Base Editing(BE)and Prime Editing(PE)based on the CRISPR/Cas9 system allow site-specific gene mutations without a DNA template.Currently,CRISPR/Cas9 gene editing technology had made significant progress in the improvement of pig production traits and the creation of new materials for disease-resistant breeding,showing enormous development potential and application prospects.This article focused on the introduction of CRISPR/Cas9,base editing and prime editing technologies and their research progress in pig breeding.It also looked forward to the opportunities and chal-lenges faced by pig genome editing breeding.
万方数据
维普
