摘要
目的:分析血清外泌体has_circ_0060937与非小细胞肺癌(non-small cell lung cancer, NSCLC)转移和不良预后的关系。方法:选择2017年5月至2021年12月我院诊治的63例NSCLC患者和83例良性肺结节患者作为对象。实时荧光定量PCR法检测血清外泌体has_circ_0060937表达水平，随访患者总生存率。结果:NSCLC组血清外泌体has_circ_0060937表达水平高于良性肺结节组(P<0.001)，可用于良好区分NSCLC组vs.良性结节组，受试者工作特征曲线下面积(AUC)为0.858(95%CI：0.818~0.898)。根据血清外泌体has_circ_0060937表达水平中位值对NSCLC患者分层，高表达亚组TNM分期、淋巴结转移和远处转移>2个位置的风险高(P<0.05)。血清外泌体has_circ_0060937表达水平预测NSCLC淋巴结转移或远处转移>2个位置的AUC分别为0.855(95%CI：0.807~0.903)和0.762(95%CI：0.702~0.823)。血清外泌体has_circ_0060937低表达亚组总生存预后更长(χ2＝14.022，P<0.001)。Cox回归分析显示血清外泌体has_circ_0060937表达水平(HR＝1.375，P<0.001)是不良总体生存的显著预测因子。结论:血清外泌体has_circ_0060937有望成为NSCLC转移和不良预后的新型生物标志物。
Abstract
Objective:To investigate the relationship between serum exosomal has_circ_0060937 and metastasis and poor prognosis of non-small cell lung cancer (NSCLC).Methods:From May 2017 to December 2021, 63 patients with NSCLC and 83 patients with benign pulmonary nodulestreated in our hospital were included as the study subjects. The expression level of serum exosomal has_circ_0060937 was detected by real-time fluorescence quantitative PCR, and the overall survival rate was followed up.Results:The expression level of serum exosomal has_circ_0060937 in NSCLC group was significantly higher than that in benign pulmonary nodules group(P<0.001), which can be used to distinguish NSCLC group vs. benign pulmonary nodules group. The area under the subject operating characteristic curve (AUC) was 0.858(95%CI: 0.818~0.898) and NSCLC patients were stratiized according to the median expression level of serum exosomal has_circ_0060937, and the high expression subgroup had a higher risk of TNM staging, lymph node metastasis and distant metastasis >2 locations (P<0.05). Serum exosomal has_circ_0060937 expression level predicted that the AUC of NSCLC lymph node metastasis or distant metastasis >2 sites was 0.855(95%CI: 0.807~0.903) and 0.762(95%CI: 0.702~0.823), respectively. The subgroup with low expression of serum exosomal has_circ_0060937 had a longer overall survival prognosis (χ2=14.022, P<0.00). Cox regression analysis showed that serum exosomal has_circ_0060937 expression level (HR=1.375, P<0.001) was a significant independent predictor of adverse overall survival.Conclusion:Serum exosomal has_circ_0060937 is expected to be a new reliable biomarker for predicting metastasis and poor prognosis of NSCLC.
基金项目
重庆市科卫联合医学科研项目(2021MSXM20)
万方数据