摘要
目的:分析Holliday交叉识别蛋白(holliday junction recognition protein, HJURP)在肺腺癌(lung adenocarcinoma, LUAD)中的表达水平及其在预后评估中的价值。方法:从公共数据库TCGA(The Cancer Genome Atlas)和GEO(Gene Expression Omnibus)下载LUAD患者的基因表达和临床数据，利用R语言软件分析HJURP的表达水平；从HPA(Human Protein Atlas)数据库下载LUAD患者的HJURP蛋白表达数据；应用ssGSEA算法进行免疫浸润分析；ROC曲线(receiver operating characteristic curve)、Kaplan-Meier分析、单因素和多因素COX回归分析、列线图和校准曲线评价HJURP在肿瘤预后评估中的价值。结果:LUAD中HJURP的表达水平显著高于癌旁组织(P<0.001)；免疫浸润分析结果显示HJURP与嗜酸性粒细胞、肥大细胞富集评分呈负相关(P<0.001)，与活化CD4 T细胞富集评分呈正相关(P<0.001)；在预后分析结果中，HJURP对LUAD预后灵敏度高，HJURP高表达的患者总生存率较差。COX回归分析显示，HJURP表达是总生存率的风险因素。结论:HJURP在LUAD中表达异常上调，并与不良预后显著相关，提示HJURP可能为LUAD潜在的诊断标志物和治疗靶点。
Abstract
Objective:To investigate the expression leve land prognostic significance of Holliday cross recognition protein (HJURP) in lung adenocarcinoma (LUAD).Methods:Gene expression and clinical data of LUAD patients were downloaded from the public databases TCGA (The Cancer Genome Atlas) and GEO (Gene Expression Omnibus), the expression levels of HJURP were analyzed by R language software. HJURP protein expression date of LUAD patients were downloaded from the HPA (Human Protein Atlas). SsGSEA algorithm was used to analyze the immune infiltration. Then, ROC curves, Kaplan-Meier analysis, univariate and multivariate COX regression analysis, nomograms and calibration curves were applied to evaluate the association between HJURP expression and clinical prognosis.Results:The expression of HJURP was significantly higher in LUAD than in adjacent tissues (P<0.001). Immune filtration analysis showed that HJURP was negatively correlated with eosinophils and mastcells(P<0.001), and positively correlated with activated CD4 T cells (P<0.001). In the results of the clinical prognosis analysis, HJURP was highly sensitive to the prognosis of LUAD, and patients with high HJURP expression had poor overall survival. COX regression analysis showed that the expression of HJURP was an independent risk factor for the overall survival rate.Conclusion:HJURP is overexpressed in LUAD and is significantly associated with poor prognosis, suggesting the potential of HJURP as a diagnostic marker and therapeutic target for LUAD.
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