摘要
目的 探讨念珠菌黏附性与生物膜形成的关系。方法 将8株念珠菌和1株酿酒酵母均分别在YPD液体培养基和琼脂培养基中培养，观察其絮凝沉淀和黏附现象，进一步在96孔微量培养皿中建立生物膜模型，测定生物膜的生长动力学变化。结果 9株菌株均具有絮凝沉淀的能力，经轻微振荡后白念珠菌、乳酒念珠菌、光滑念珠菌和热带念珠菌不易重新悬浮，而酿酒酵母容易重新悬浮。9株菌株均能黏附于YPD琼脂培养基的表面，白念珠菌、乳酒念珠菌、光滑念珠菌和热带念珠菌的黏附性强，而其他菌株的黏附性弱。常见的致病性念珠菌都能形成生物膜，且生物膜念珠菌活性随培养时间延长而增加。白念珠菌和乳酒念珠菌形成生物膜的能力较其他菌株强(P＜0.05)；热带念珠菌和光滑念珠菌形成生物膜的能力较克柔念珠菌、近平滑念珠菌和高里念珠菌强(P＜0.05)。不致病的酿酒酵母不能形成生物膜。结论 念珠菌具有黏附的能力，且强弱不同。致病性念珠菌具有形成生物膜的能力，且强弱不同。念珠菌形成生物膜的能力与其黏附力呈正相关。
Abstract
Objective To assess the relationship between Candida adhesivity and biofilm formation. Methods Eight Candida strains belonging to 8 species and 1 Saccharomyces cerevisiae strain were cultured in yeast peptone dextrose (YPD) fluid and agar medium respectively. The flocculation and adhesion of Candida were observed. Candida biofilm models were developed in 96-well microculture plates. The kinetics of biofilm formation was measured. Results All the 9 fungal strains had flocculation capability and could adhere to the surface of the yeast peptone dextrose agar medium. After mild shaking of the fluid medium, it is difficult for C. albicans, C. kefyr, C. glabrata and C. tropicalis to resuspend, but easy for Saccharomyces cerevisiae. The adhesivity of C. albicans, C. kefyr, C. glabrata and C. tropicalis was stronger than that of the other Candida strains. Common pathogenic Candida strains could form biofilm, and the metabolic activity of Candida cells in the biofilm increased over time. A significant increment was observed in the ability of C. albicans and C. kefyr to form biofilm compared with the other species (all P ＜ 0.05), and in that of C. tropicalis and C. glabrata compared with C. krusei, C. parapsilosis and C. gulliermondii (all P ＜ 0.05). The nonpathogenic Saccharomyces cerevisiae could not form biofilm. Conclusions Candida has the ability to adhere and form biofilm,and the ability varies with Candida species. Moreover, the ability to form biofilm positively correlates with the adhesivity of Candida.
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维普
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