核纤层蛋白B2通过调控AKT信号通路促进腹膜后脂肪肉瘤细胞的迁移
Lamin B2 promotes migration of retroperitoneal liposarcoma cells by regulating AKT signaling pathway
马世祥 1肖萌萌 1李向冀 2罗成华1
作者信息
- 1. 北京大学国际医院腹膜后肿瘤外科,北京 102206
- 2. 首都医科大学附属北京友谊医院消化内科,北京 100050
- 折叠
摘要
目的 探究核纤层蛋白B2(LMNB2)对腹膜后脂肪肉瘤细胞(SW872)迁移能力的影响及其作用机制.方法 采用免疫组化实验分析33例腹膜后脂肪肉瘤患者肿瘤组织样本中LMNB2表达水平的差异,分析LMNB2表达水平与患者预后及临床病理特征的关系.转染siRNA降低肿瘤细胞中LMNB2的表达,通过细胞划痕实验和Transwell迁移实验检测LMNB2对SW872细胞体外划痕愈合及迁移能力的影响.Western blot检测p-AKT和AKT蛋白在各组细胞中的表达水平.结果 LMNB2高表达组患者的无复发生存率和总体生存率比低表达组更低,并且更容易复发,差异均具有统计学意义(x2=4.872,P=0.027;x2=4.180,P=0.041;x2=7.127,P=0.008).伴随着 LMNB2表达的沉默,细胞的迁移能力明显降低(t=11.240,P＜0.01;t=7.445,P＜0.01).沉默组细胞中p-AKT表达水平明显低于对照组,而AKT蛋白的表达差异无统计学意义(t=9.784,P＜0.01).结论 LMNB2可能通过调控AKT信号通路促进腹膜后脂肪肉瘤细胞SW872的迁移能力.
Abstract
Objective To investigate the effect of Lamins B2(LMNB2)on the migration of human retroperitoneal liposarcoma(RPLS)cells SW872.Methods Immunohistochemistry was used to analyze the the differential expression levels of LMNB2 in 33 RPLS tissue samples.The correlation between LMNB2 expression and clinical prognosis and clinicopathological features was analyzed.siRNA was used to lower the expression level of LMNB2 in tumor cells,and the effect of LMNB2 on the scratch healing ability and migration ability of SW872 cells was examined by using wound-healing assay and transwell migration assay.The expression levels of p-AKT and AKT in each group cells were detected by Western blot Results Patients with high LMNB2 expression had a lower recurrence-free survival and overall survival compared to those with low LMNB2 expression,and were more likely to experience recurrence,(x2=4.872,P=0.027;x2=4.180,P=0.041;x2=7.127,P=0.008).The migration ability of cells was significantly reduced following the silencing of LMNB2 expression(t=11.240,P＜0.01;t=7.445,P＜0.01).The expression level of p-AKT in the silencing group was significantly lower than that in the control group,while there was no significant difference in the expression level of AKT between the two groups(t=9.784,P＜0.01).Conclusion LMNB2 may promote the migration of human retroperitoneal liposarcoma cells SW872 by regulating AKT signaling pathway.
关键词
脂肪肉瘤/细胞迁移分析/基因表达调控Key words
Liposarcoma/Cell migration assays/Gene expression regulation引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据