EPSTI1通过调节AKT活化促进胰腺癌细胞迁移、侵袭和上皮间质转化
EPSTI1 promotes migration,invasion and epithelial mesenchymal transformation of pancreatic cancer cells by regulating AKT activation
徐杰 1袁帅 1徐培钧 2高松2
作者信息
- 1. 天津医科大学肿瘤医院高级病房 国家恶性肿瘤临床医学研究中心 天津市恶性肿瘤临床医学研究中心 天津市肿瘤防治重点实验室,天津 300060
- 2. 天津医科大学肿瘤医院胰腺肿瘤科 国家恶性肿瘤临床医学研究中心 天津市恶性肿瘤临床医学研究中心 天津市肿瘤防治重点实验室,天津 300060
- 折叠
摘要
目的 探讨上皮基质相互作用蛋白1(epithelial stromal interaction 1,EPSTI1)在胰腺癌发生发展过程中对癌细胞迁移、侵袭和上皮间质转化的作用.方法 对132例胰腺癌组织样本进行免疫组化染色评估,对体外培养的癌细胞进行转染,采用Western blot、Transwell试验分析EPSTI 1对胰腺癌细胞迁移、侵袭和上皮间质转化的影响.结果 EPSTI1在胰腺癌细胞中的异常高表达可导致癌细胞的上皮-间质转化(epithelial-mesenchymal transition,EMT),增加胰腺癌细胞的迁移和侵袭(均P＜0.05).通过与胰腺星形细胞共培养,癌细胞中EPSTI1表达显著升高.EPSTI1与磷酸化蛋白激酶(phosphorylated protein kinase,AKT)相互作用,可诱导AKT磷酸化,促进上述恶性表型(均P＜0.05).结论 EPSTI 1部分通过调节AKT激活促进胰腺癌细胞迁移、侵袭和EMT.
Abstract
Objective To investigate the role of epithelial stromal interaction 1(EPSTI1)in cancer cell migration,invasion and epithelial mesenchymal transformation in the pathogenesis of pancreatic cancer.Methods Immunohistochemical staining was performed on the 132 pancreatic cancer tissue samples.Transfection of cancer cells was cultured in vitro.Western blot,transwell experiments were used to analyze the effects of EPSTI1 on migration,invasion and epithelial mesenchymal transformation of pancreatic cancer cells.Results Abnormally high expression of EPSTI1 in pancreatic cancer cells could lead to epithelial-mesenchymal transition(EMT)and increase migration and invasion of pancreatic cancer cells.After co-culture with pancreatic astrocytes,EPSTI1 expression in cancer cells was significantly increased(P＜0.05).EPSTI1 interacts with phosphorylated protein kinase(AKT)to induce phosphorylation of AKT and promote malignant phenotype(P＜0.05).Conclusion EPSTI1 promotes migration,invasion and EMT of pancreatic cancer cells possibly by regulating AKT activation.
关键词
胰腺肿瘤/上皮细胞-间充质细胞转换/上皮基质相互作用蛋白1/磷酸化蛋白激酶Key words
Pancreatic neoplasms/Epithelial-mesenchymal transition/Epithelial stromal interaction 1/Phosphorylated protein kinase引用本文复制引用
基金项目
国家自然科学基金(82072657)
天津市医学重点学科(与科)建设项目(TJYXZDXK-009A)
出版年
2024
NETL
NSTL
维普
万方数据