摘要
目的:探讨转录因子ELK3（ELK3）在胃癌组织和癌旁组织中的表达与临床病理特征及预后的关系。方法:选取2019年1月至2021年6月行手术治疗的60例胃癌患者癌组织和对应癌旁组织（距肿瘤边缘3~5 cm）标本进行研究。采用免疫组织化学染色法检测胃癌组织及癌旁组织中ELK3表达情况。采用SPSS 21.0软件分析数据，计数资料以例表示，组间比较采用χ2检验；Kaplan-Meier生存函数分析ELK3与患者预后的关系；Cox回归模型分析影响胃癌患者预后生存的危险因素。P<0.05表示差异有统计学意义。结果:胃癌组织中ELK3阳性表达率显著高于癌旁组织（χ2=11.422，P=0.001）；胃癌组织中ELK3阳性表达率与淋巴结转移、肿瘤分期、分化程度、病理分型显著相关（P<0.05）；60例胃癌患者均获得随访，中位随访时间25个月。ELK3阳性组患者累积总生存率显著低于ELK3阴性组（Log-Rank χ2=4.192，P=0.041）；淋巴结转移、肿瘤分期、分化程度、神经侵犯、术后放化疗、ELK3阳性表达是影响胃癌患者预后的独立危险因素（P<0.05）。结论:胃癌组织中ELK3显著阳性表达，与淋巴结转移、肿瘤分期、分化程度、病理分型等病理特征及患者预后关系密切，是影响胃癌患者预后的独立危险因素，可能成为胃癌预后评估的潜在生物标志物。
Abstract
Objective:To analyze the relationship between the expression of transcription factor ELK3 (ELK3) and the clinicopathologic features and prognosis in gastric and paracancer tissues.Methods:Samples of cancer tissues and corresponding paracancer tissues (3~5 cm away from tumor margin) from 60 patients with gastric cancer who underwent surgical treatment from January 2019 to June 2021 were selected for study. The expression of ELK3 in gastric cancer tissues and adjacent tissues was detected by immunohistochemical staining. SPSS 21.0 software was used to analyze the data, and the count data were presented as examples. χ2 test was used for comparison between groups. Kaplan-Meier survival function was used to analyze the relationship between ELK3 and prognosis. Cox regression model was used to analyze the risk factors affecting prognosis and survival of patients with gastric cancer. P<0.05 meant the difference was statistically significant.Results:The positive expression rate of ELK3 in gastric cancer tissues was significantly higher than that in paracancer tissues (χ2=11.422, P=0.001). The positive expression rate of ELK3 in gastric cancer tissues was significantly correlated with lymph node metastasis, tumor stage, degree of differentiation and pathological classification (P<0.05). All 60 patients with gastric cancer were followed up for a median of 25 months.The cumulative overall survival rate of ELK3-positive group was significantly lower than that of ELK3-negative group (Log-Rank χ2=4.192, P=0.041). Lymph node metastasis, tumor stage, degree of differentiation, nerve invasion, postoperative chemoradiotherapy and positive expression of ELK3 were independent risk factors affecting the prognosis of gastric cancer patients (P<0.05).Conclusion:The significant positive expression of ELK3 in gastric cancer tissues is closely related to pathological characteristics such as lymph node metastasis, tumor stage, differentiation degree, pathological type and patient prognosis. It is an independent risk factor affecting the prognosis of cancer patients, and may become a potential biomarker for the prognosis evaluation of gastric cancer.
基金项目
江苏省卫生健康委2018年度科研项目(H2018091)
万方数据