摘要
目的:分析肝门部胆管癌（HC）组织中微小RNA-98-5p（mir-98-5p）、ALKB同源蛋白1（ALKBH1）表达水平，并探讨其与患者临床病理特征及预后的关系。方法:选取2011年6月至2017年6月行根治性手术的96例HC患者癌组织和相对应的癌旁正常组织（≥5 cm）为研究对象。实时荧光定量PCR（qRT-PCR）法检测组织中mir-98-5p、ALKBH1 mRNA表达水平，免疫组织化学法检测组织中ALKBH1表达。用SPSS 25.0对数据进行统计学分析，计量资料以（）表示，行独立样本t检验；计数资料行χ2检验；使用Pearson相关分析HC组织中mir-98-5p与ALKBH1 mRNA表达水平的关系；使用Kaplan-Meier进行生存曲线分析mir-98-5p、ALKBH1与HC患者5年内总生存率的关系。P<0.05为差异有统计学意义。结果:与癌旁正常组织相比，HC组织中ALKBH1 mRNA相对表达水平、ALKBH1阳性率较高（P<0.05），mir-98-5p相对表达水平较低（P<0.05）；mir-98-5p、ALKBH1与HC患者淋巴结转移、TNM分期相关（P<0.05）。Pearson相关分析显示，HC组织中mir-98-5p与ALKBH1 mRNA表达水平呈负相关（P<0.05）。Kaplan-Meier生存分析显示，mir-98-5p低表达组5年累计生存率为18.8%，低于mir-98-5p高表达组33.3%（P<0.05）；ALKBH1阳性组5年累计生存率为14.0%，低于ALKBH1阴性组43.6%（P<0.05）。结论:mir-98-5p低表达、ALKBH1高表达与HC患者淋巴结转移、TNM分期及预后相关。
Abstract
Objective:The expression levels of microrNA-98-5p (mir-98-5p) and ALKB homologous protein 1 (ALKBH1) in hilar cholangiocarcinoma (HC) were analyzed, and the relationship between microrNA-98-5P and clinicopathological features and prognosis of patients was discussed.Methods:Cancer tissues and corresponding adjacent normal tissues (≥5 cm) of 96 patients with HC who underwent radical surgery from June 2011 to June 2017 were selected as the study objects. Real-time quantitative fluorescent PCR (qRT-PCR) was used to detect the mRNA expression levels of mir-98-5p and ALKBH1 in all tissues, and immunohistochemistry was used to detect the expression levels of ALKBH1 in tissues. Statistical analysis was performed using SPSS 25.0, the measurement data were expressed as (), independent sample t test was performed, and χ2 test was performed for comparison among the count groups. Pearson correlation was used to analyze the relationship between mir-98-5p and ALKBH1 mRNA expression levels in HC tissues. Kaplan-Meier survival curve was used to analyze the relationship between mir-98-5p, ALKBH1 and 5-year overall survival of HC patients. P<0.05 was considered statistically significant.Results:The relative expression level of ALKBH1 mRNA and positive rate of ALKBH1 in HC tissues were higher than those in adjacent normal tissues (P < 0.05), while the relative expression level of mir-98-5p was lower in HC tissues (P < 0.05). mir-98-5p and ALKBH1 were correlated with lymph node metastasis and TNM stage in HC patients (P < 0.05). The results of Pearson correlation analysis showed that mir-98-5p was negatively correlated with ALKBH1 mRNA expression in HC tissues (P < 0.05). Kaplan-Meier survival analysis showed that the 5-year cumulative survival rate of mir-98-5p low expression group was 18.8%, which was lower than that of mir-98-5p high expression group (33.3%) (P < 0.05). The 5-year cumulative survival rate of ALKBH1-positive group was 14.0%, and that of AlkBH1-negative group was 43.6% (P < 0.05).Conclusion:Low expression of mir-98-5p and high expression of ALKBH1 were correlated with lymph node metastasis, TNM stage and prognosis of HC patients.
基金项目
内蒙古自治区自然科学重点项目(NJZZ22654)
万方数据