Influences and mechanism of extracellular vesicles from dermal papilla cells of mice on human hypertrophic scar fibroblasts
Objective To investigate the influences and mechanism of extracellular vesicles from dermal papilla cells(DPC-EVs)of mice on human hypertrophic scar fibroblasts(HSFs).Methods The study was an experimental research.The primary dermal papilla cells(DPCs)of whiskers were extracted from 10 6-week-old male C57BL/6J mice and identified successfully.The DPC-EVs were extracted from the 3rd to 5th passage DPCs by ultracentrifugation,and the morphology was observed through transmission electron microscope and the particle diameter was detected by nanoparticle tracking analyzer(n=3)at 24 h after culture.The 3rd passage of HSFs were divided into DPC-EV group and phosphate buffer solution(PBS)group,which were cultured with DPC-EVs and PBS,respectively.The cell scratch test was performed and cell migration rate at 24 h after scratching was calculated(n=5).The cell proliferation levels at 0(after 12 h of starvation treatment and before adding DPC-EVs or PBS),24,48,72,and 96 h after culture were detected by using cell counting kit 8(n=4).The protein expressions of α-smooth muscle actin(α-SMA)and collagen type Ⅰ(Col Ⅰ)in cells at 24 h after culture were detected by immunofluorescence method and Western blotting,and the protein expression of Krüppel-like factor 4(KLF4)in cells at 24 h after culture was detected by Western blotting.After the 3rd passage of HSFs were cultured with DPC-EVs for 24 h,the cells were divided into blank control group,KLF4 knockdown group,and KLF4 overexpression group according to the random number table.The cells in blank control group were only routinely cultured for 48 h.The cells in KLF4 knockdown group and KLF4 overexpression group were incubated with KLF4 knockdown virus for 24 h,then the cells in KLF4 knockdown group were routinely cultured for 24 h while the cells in KLF4 overexpression group were incubated with KLF4 overexpression virus for 24 h.The protein expressions of KLF4,α-SMA,and Col Ⅰ in cells were detected by Western blotting at 48 h after culture.Results At 24 h after culture,the extracted DPC-EVs showed vesicular structure with an average particle diameter of 108.8 nm.At 24 h after scratching,the migration rate of HSFs in PBS group was(54±10)％,which was significantly higher than(29±8)％in DPC-EV group(t=4.37,P＜0.05).At 48,72,and 96 h after culture,the proliferation levels of HSFs in DPC-EV group were significantly lower than those in PBS group(with t values of 4.06,5.76,and 6.41,respectively,P＜0.05).At 24 h after culture,the protein expressions of α-SMA and Col Ⅰ of HSFs in DPC-EV group were significantly lower than those in PBS group,while the protein expression of KLF4 was significantly higher than that in PBS group.At 48 h after culture,compared with those in blank control group,the protein expression of KLF4 of HSFs in KLF4 knockdown group was down-regulated,while the protein expressions of α-SMA and Col Ⅰ were both up-regulated;compared with those in KLF4 knockdown group,the protein expression of KLF4 of HSFs in KLF4 overexpression group was up-regulated,while the protein expressions of Col Ⅰ andα-SMA were down-regulated.Conclusions The DPC-EVs of mice can inhibit the proliferation and migration of human HSFs and significantly inhibit the expressions of fibrosis markers α-SMA and ColⅠ in human HSFs by activating KLF4.
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