中国罕见A组轮状病毒DS-1样G3P[8]的全基因组分子特征分析
Genome-wide molecular characterization of a rare group A rotavirus equine-like DS-1-like G3P[8]in China
熊光萍 1魏宇航 2彭蕊 3范佳欣 3唐晓苹 1黄枝妙 4董梦洁 3车如意 3李丹地3
作者信息
- 1. 甘肃中医药大学公共卫生学院,兰州 730000;中国疾病预防控制中心病毒病预防控制所国家卫生健康委员会医学病毒和病毒病重点实验室,北京 102206
- 2. 中国疾病预防控制中心病毒病预防控制所国家卫生健康委员会医学病毒和病毒病重点实验室,北京 102206;北京市通州区卫生健康监督所,北京 101100
- 3. 中国疾病预防控制中心病毒病预防控制所国家卫生健康委员会医学病毒和病毒病重点实验室,北京 102206
- 4. 福建省疾病预防控制中心,福州 350012
- 折叠
摘要
目的 对2021年采集于我国福建的A组轮状病毒(group A rotavirus,RVA)G3P[8]毒株FJ21351116进行全基因组分子特征分析.方法 使用高灵敏度A组轮状病毒全基因组测序方法对FJ21351116进行全基因组测序.用MEGA11.0、Geneious9.0.2和DNASTAR软件通过核酸序列分析评估病毒的基因组特征.使用BioEdit v.7.0.9.0和PyMOL v.2.5.2分析VP7和VP4(VP8*)的中和表位.结果 我国福建RVA毒株FJ21351116基因型为G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2,系统进化分析显示,FJ21351116株的VP7、VP4、VP3、NSP2-NSP5基因与近几年日本检测到的马样DS-1样G3P[8]基因存在亲缘关系.而VP6、VP1、VP2、NSP1基因与大部分国家G2P[4]的相应基因亲缘关系近,特别是新加坡,表明该毒株是马样G3P[8]毒株与G2P[4]毒株共感染过程中通过基因重配形成的.FJ21351116的VP7/VP4基因与Rotarix和RotaTeq疫苗的进化分析表明,VP7和VP4(VP8*)中和抗原表位与疫苗氨基酸位点均存在多个突变.推测Rotarix和RotaTeq疫苗针对马样DS-1样G3P[8]RVA的保护效果不佳,且与Rotarix的中和抗原表位氨基酸差异高于RotaTeq.结论 本研究发现一例中国罕见的DS-1样G3P[8]型RVA毒株,且疫苗株可能对其保护效果差,强调了持续监测RVA毒株及研发高效覆盖面全的RVA疫苗的重要性.
Abstract
Objective A genome-wide molecular characterization of FJ21351116,a strain of G3P[8]-E2 2021 collected in Fujian,China,was performed.Methods Whole genome sequencing of FJ21351116 was performed using a high-sensitivity group A rotavirus whole genome sequencing method.Genomic characteriza-tion of the virus was assessed by nucleic acid sequence analysis using MEGA 11.0,Geneious 9.0.2 and DNASTAR software.Neutralization epitopes of VP7 and VP4(VP8*)were analyzed using BioEdit v.7.0.9.0 and PyMOL v.2.5.2.Results In this study,FJ21351116 was shown to be a G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genotype,and the result of phylogenetic tree showed that the VP7,VP4,VP3,and NSP2-NSP5 genes of the FJ21351116 strain were related to the equine-like DS-1-like G3P[8]genes that have been detected in Japan in recent years.VP6,VP1,VP2,and NSP1 genes are closely related to G2P[4]in most countries,especially in Singapore,suggesting that this strain was formed by genetic reassortment during the evolution of equine-like G3P[8]and G2P[4].Evolutionary relationships between the VP7/VP4 genes of FJ21351116 and Rotarix and RotaTeq vaccines suggest that the multiple mutations in both VP7 and VP4(VP8*)neutralizing antigenic epitopes and vaccine amino acid sites.It is hypothesized that the Rotarix and RotaTeq vaccines may be less effective against equine DS-1-like G3P[8]RVA,and the sequence differences with Rotarix are higher than those with RotaTeq.Conclusions In this study,we found a rare case of DS-1-like G3P[8]RVA strain in China.Currently,horse-like DS-1-like G3P[8]RVA is relatively rare in China and may be poorly protected by vaccine strains,emphasizing the importance of continuous monitoring of RVA strains and the development of efficient and full-coverage RVA vaccines.
关键词
A组轮状病毒/全基因组/DS-1-like/G3P[8]/分子特征Key words
Group A rotavirus/Genome-wide/DS-1-like G3P[8]/Molecular characterization引用本文复制引用
基金项目
国家重点研发计划(2022YFC2304302)
国家重点研发计划(2018YFC1200602)
国家自然科学基金(21934005)
出版年
2024
NETL
NSTL
万方数据