2022-2023年福建省监测点环境污水中诺如病毒的检测和型别分布特征分析
Analysis on the detection and genotypes distribution of norovirus in environmental sewage in Fujian province during 2022-2023
严诗琦 1张梦萍 2张海荣 2吴冰珊 2李东 2陈致飞 2杨秀惠1
作者信息
- 1. 福建医科大学公共卫生学院,福州 350001;福建省疾病预防控制中心福建省人兽共患病重点实验室,福州 350010
- 2. 福建省疾病预防控制中心福建省人兽共患病重点实验室,福州 350010
- 折叠
摘要
目的 了解福建省3个监测点环境污水中诺如病毒(norovirus,NoV)检出情况及型别分布特征,探索其应用于NoV监测的意义.方法 每月在代表性监测地区5个采样点采集污水,富集浓缩后,用逆转录半巢式-聚合酶链反应(reverse transcription-semi nested polymerase chain reaction,RT-snPCR)扩增NoV VP1部分基因片段,TA克隆后测序进行基因型别分析.结果 2022年7月至2023年6月共收集污水标本56份,G Ⅰ和GⅡ检出率分别为89.29％(50/56)和94.64％(53/56).检出GⅠ组7种基因型和GⅡ组13种基因型,其中GⅠ.1、GⅠ.4、GⅡ.4和GⅡ.17为优势基因型.不同采样点检出基因型别不完全一致;2022年8～11月NoV检出率较低,且不同季节流行优势基因型不同,GⅠ.1和GⅡ.4在2022年8～11月呈高流行,但2022年12月到2023年6月分别被G Ⅰ.4和GⅡ.17取代.2023年1～6月相较2022年7～12月检出型别丰度更高.优势基因型GⅡ.17具有多进化分支,发现了不同于2014/2015年流行株的新变异株.结论 环境污水NoV检出率高且基因型别多样,具备发现新变异病毒株的能力,环境污水监测可做为NoV病例监测的重要补充手段.
Abstract
Objective To investigate the detection status and genotypes distribution characteristics of norovirus(NoV)in environmental sewage from three monitoring points in Fujian province,and to explore the significance of its application to NoV monitoring.Methods Sewage samples were collected monthly at 5 sampling sites in representative monitoring cities,enriched and concentrated.Partial gene fragments of norovirus VP1 were amplified by reverse transcription-semi nested polymerase chain reaction(RT-snPCR),TA cloned and sequenced.Genotypes were identified based on the sequencing.Results A total of 56 sewage samples were collected from July 2022 to June 2023.The detection rates of G Ⅰ and G Ⅱ were 89.29％(50/56)and 94.64％(53/56),respectively.A total of 7 NoV G Ⅰ genotypes and 13 G Ⅱgenotypes were identified.GⅠ.1,G Ⅰ.4,G Ⅱ.4 and G Ⅱ.17 were the dominant genotypes.NoV genotypes detected in different sampling sites were not exactly the same.The detection rate of NoV was low from August to November 2022,and the prevalence of the dominant genotypes was different in different seasons.GⅠ.1 and G Ⅱ.4 were highly prevalent from August to November 2022,but were replaced by GⅠ.4 and G Ⅱ.17 from December 2022 to June 2023,respectively.More NoV genotypes were detected in January-June 2023,comparing to the July-December 2022.The dominant genotype GⅡ.17,has multiple clades and new variants have been discovered that are different from the 2014/2015 circulating strains.Conclusions The detection rates of NoV in environmental sewage were very high,and genotypes were diverse.Environmental sewage surveillance could be an important complementary method for NoV cases surveillance.
关键词
环境监测/诺如病毒/污水/基因型Key words
Environmental surveillance/Norovirus/Sewage/Genotype引用本文复制引用
基金项目
福建省社会发展引导性重点项目(2017Y0011)
福建省科技平台建设项目(2019Y2001)
出版年
2024
NETL
NSTL
万方数据