一株GⅡ.12[P16]型诺如病毒毒株的全基因组序列分析
Analysis of the whole genome sequence of a G Ⅱ.12[P1 6]norovirus strain
李美佳 1王国强 2郭明鑫 1刘晓林 3刘倜 3孙文魁 3傅忠燕 3寇增强4
作者信息
- 1. 山东第一医科大学(山东省医学科学院)公共卫生与健康管理学院,济南 250117
- 2. 德州市疾病预防控制中心检验科,德州 253058
- 3. 山东省疾病预防控制中心传染病防制所,济南 250014
- 4. 山东第一医科大学(山东省医学科学院)公共卫生与健康管理学院,济南 250117;山东省疾病预防控制中心传染病防制所,济南 250014
- 折叠
摘要
目的 对一株诺如病毒分离株SD20200267进行鉴定和全基因组序列分析,了解其结构特征.方法 取患者标本进行病毒核酸的提取,对样本核酸进行扩增,将扩增产物进行测序,根据核苷酸序列进行基因分型.采用宏基因组测序技术进行全基因组测序,对测序所得的核苷酸序列进行分析.结果 获得SD20200267株病毒基因组全长,总长度为7 465个核苷酸.SD20200267株在VP1区及RdRp区分别属于GⅡ.12和GⅡ.P16基因型.SD20200267株与其他GⅡ.12[P16]型毒株核苷酸一致性在96.0％～97.3％之间,有15个氨基酸位点变异.该毒株存在重组现象,重组位点位于ORF1与ORF2重叠区域.结论 SD20200267株为GⅡ.12[P16]型毒株,ORF1与ORF2重叠区域存在重组现象.
Abstract
Objective To characterize the complete genome sequence and elucidate the structural features of norovirus(NoV)isolate SD20200267.Methods The viral nucleic acid was extracted from patient samples,followed by amplification and sequencing for genotyping based on the nucleotide sequences.The metagenomic sequencing technology was utilized for whole genome sequencing,and subsequent analysis was performed on the acquired nucleotide sequences.Results The complete genome sequence of the SD20200267 strain,spanning a total length of 7 465 nucleotides,was successfully obtained.The SD20200267 strain belongs to the G Ⅱ.12 and G Ⅱ.P16 genotypes in the VP1 and RdRp regions,respectively.The nucleotide sequence identity of SD20200267 strain with other G Ⅱ.12[P16]strains ranged from 96.0％to 97.3％,exhibiting 15 amino acid variations.The strain displayed evidence of recombination,with the recombination site located in the overlapping region of ORF1 and ORF2.Conclusions SD20200267 is classified as a G Ⅱ.12[P16]strain,and recombination was observed in the overlapping region of ORF1 and ORF2.
关键词
诺如病毒/基因组/重组Key words
Norovirus/Genome/Recombination引用本文复制引用
基金项目
国家重点研发计划(2021YFC2302003)
出版年
2024
NETL
NSTL
万方数据