2019-2022年铜陵市B型Victoria系流感病毒HA和NA基因进化特征分析
Evolutionary characteristics of HA and NA genes of B Victoria influenza virus in Tongling city from 2019 to 2022
张义华 1叶梦 1周马云 1李承宝 1金玲娟 1陈娟 1蔡亦红2
作者信息
- 1. 铜陵市疾病预防控制中心,铜陵 244000
- 2. 安徽医科大学公共卫生学院,合肥 230032
- 折叠
摘要
目的 分析2019-2022监测年度铜陵市B型Victoria系流感病毒血凝素(hemagglutinin,HA)和神经氨酸酶(Neuraminidase,NA)的遗传进化特征.方法 选取2019-2022年度本实验室分离的22株B型Victoria系流感病毒进行全基因组测序,利用生物信息分析软件进行序列对比和系统进化分析.结果 2019-2022年铜陵市季节性流感主要以B型Victoria系流感为主,归属于V1A.3分支,其中2021-2022年分离的14株归属V1A.3a.2分支.与疫苗株相比,22株B型Victoria系流感的HA与NA蛋白均出现多个氨基酸变异位点,HA蛋白的四个主要抗原表位(120环、150环、160和190螺旋)均有变异,NA蛋白耐药位点未检测出变异,HA蛋白197 NETQ糖基化位点也发生改变.结论 2019-2022年铜陵市B型Victoria系流感病毒的HA蛋白的氨基酸位点部分发生了变异现象,这可能导致抗原表位的漂移变化,因此需要进一步加强流感病毒变异监测.
Abstract
Objective To analyze the genetic evolution characteristics of hemagglutinin(HA)and neuraminidase(NA)of influenza B virus in Tongling during 2019-2022 surveillance years.Methods Twenty-two strains of Victoria influenza B virus isolated from our laboratory during 2019-2022 were selected for whole genome sequencing.The sequence comparison and phylogenetic analysis were conducted by using bioinformatic analysis software.Results During 2019-2022,seasonal influenza in Tongling City was predominantly caused by influenza B Victoria lineage viruses,which fell within the V1A.3 branch.Among these,14 strains isolated in the 2021-2022 season were further classified into the V1A.3a.2 sub-branch.Compared with vaccine strains,multiple amino acid mutation sites were detected in both HA and NA proteins of the 22 influenza B Victoria lineage viruses.Notably,all four major antigenic sites(120-loop,150-loop,160-helix,and 190-helix regions)in the HA protein exhibited variations.Although no mutations were detected at resistance sites on the NA protein,a change occurred in the glycosylation site at position 197 NETQ in the HA protein.Conclusions The main amino acid sites of the HA protein of the influenza B Victoria lineage viruses in Tongling City from 2019 to 2022 have undergone significant variation,which may lead to antigenic drift.Therefore,it is essential to strengthen the monitoring of influenza virus mutations.
关键词
B型流感/Victoria系/抗原表位/变异Key words
Influenza B/Victorians/Epitope/Variation引用本文复制引用
基金项目
安徽医科大学博士基金(XJ202005)
安徽省高等学校自然科学研究重点项目(KJ2020A0153)
出版年
2024
NETL
NSTL
万方数据