微波对模拟感染性废物中腺病毒衣壳完整性及Hexon蛋白抗原性的影响
Effect of microwave on adenovirus capsid integrity and antigenicity of hexon protein in simulated infectious wastes
王扩 1丁淑军 2李丹迪 2杨晓斌 1秦泽明 1赵丽 1唐浏英3
作者信息
- 1. 山东大学齐鲁医学院公共卫生学院微生物检验学系山东省"十三五"高等学校感染性疾病防控重点实验室,济南 250012
- 2. 山东省疾病预防控制中心山东省传染病预防控制重点实验室,济南 250014
- 3. 中国疾病预防控制中心病毒病预防控制所,北京 100052
- 折叠
摘要
目的 研究微波对模拟感染性废物中人腺病毒2型(human adenovirus,HAdV-2)衣壳蛋白的影响.方法 将HAdV-2病毒悬液滴加至医用一次性手套模拟感染性废物,在不同微波条件下对其进行辐照,记录温度变化,辐照后的病毒上清液经Dnase Ⅰ处理后通过PCR、qPCR检测病毒核酸,判断微波对病毒衣壳完整性的影响.ELISA检测微波辐照对病毒Hexon蛋白结构的影响.单独以微波辐照过程中的最高温度处理病毒,研究微波消毒过程是否存在非热效应.结果 微波辐照过程中最高温度为76℃,PCR及qPCR结果表明,Dnase Ⅰ可使微波辐照后的病毒核酸产生明显损伤,而病毒对照组及热处理组没有受到明显影响,表明微波辐照可以破坏病毒衣壳完整性.ELISA结果表明,微波辐照能使病毒Hexon蛋白与抗体结合能力明显减弱,热处理组结果与之相似.结论 微波辐照可破坏HAdV-2衣壳完整性以及Hexon蛋白结构,其中衣壳完整性受损主要是非热效应发挥作用,Hexon蛋白结构改变主要是热效应发挥作用.
Abstract
Objective To investigate the effect of microwave on human adenovirus type 2(HAdV-2)capsid protein in simulated infectious wastes.Methods Droplets of HAdV-2 virus suspension were added to medical disposable gloves to simulate infectious waste,irradiated under different microwave conditions,the temperature change was recorded,and the irradiated viral supernatant was treated with DnaseⅠ and detected by PCR and qPCR to determine the effect of microwave on the integrity of the viral capsid.ELISA was used to detect the effect of microwave irradiation on the structure of viral hexon protein.The virus was treated alone at the highest temperature during microwave irradiation to investigate whether there were non-thermal effects during microwave disinfection.Results The maximum temperature during microwave irradiation was 76 ℃,and the PCR and qPCR result showed that Dnase Ⅰ could significantly damage the viral nucleic acid after microwave irradiation,while the virus control group and heat treatment group were not significantly affected,indicating that microwave irradiation could destroy the integrity of the viral capsid.The result of ELISA showed that microwave irradiation could significantly weaken the binding ability of Hexon protein and antibody,and the result of heat treatment group were similar.Conclusions Microwave irradiation can destroy the integrity of the HAdV-2 capsid and the structure of Hexon protein,in which the damage to the integrity of the capsid is mainly due to non-thermal effects,and the structural changes of hexon protein are mainly due to thermal effects.
关键词
微波/感染性废物/腺病毒/衣壳/灭活机制Key words
Microwave/Infectious waste/Adenovirus/capsid/Inactivation mechanism引用本文复制引用
出版年
2024
NETL
NSTL
万方数据