2021-2022年绵阳地区流感样病例样本中的鼻病毒特征分析
Characterization of rhinovirus in patients with respiratory tract infection in Mianyang,2021-2022
宫悦 1潘明 2陈果 3宋芹芹 4王衍海 4高晨 4夏志强 4王璐璐 4程迁 4宗可鑫 4韩俊4
作者信息
- 1. 中国疾病预防控制中心病毒病预防控制所传染病溯源预警与智能决策全国重点实验室,北京 102206;内蒙古科技大学包头医学院,包头 014000
- 2. 四川省疾病预防控制中心,成都 610000
- 3. 绵阳市疾病预防控制中心,绵阳 621000
- 4. 中国疾病预防控制中心病毒病预防控制所传染病溯源预警与智能决策全国重点实验室,北京 102206
- 折叠
摘要
目的 了解绵阳地区的流感样病例患者的鼻病毒(human rhinovirus,HRV)流行情况及病原特征.方法 收集2021-2022年绵阳地区哨点医院呼吸道感染且诊断为流感样病例(influenza-like illness,ILIs)患者的咽拭子,采用实时荧光定量PCR技术对16种常见病原体进行检测.巢氏PCR扩增HRV阳性样本的VP4/VP2编码区基因.从GenBank数据库下载国内外各血清型代表株进行系统发育、一致性及氨基酸变异分析.结果 共采集绵阳地区ILIs样本332例,病毒检出率为58.73％(195/332).其中HRV阳性样本23例,检出率为6.92％(23/332),成功扩增出18条HRV流行株序列.经比对发现绵阳地区ILIs病例流行的HRV涉及13种血清型,分属于HRV-A(12种)、HRV-B(5种)和HRV-C(1种)3种基因型.同源性分析表明其核苷酸和氨基酸相对保守,但与参考株相比,HRV-A型和HRV-B型存在多处变异.结论 HRV是2021-2022年绵阳地区ILI感染的病原体之一,以HRV-A型为主.
Abstract
Objective To understand the prevalence and characteristics of Rhinovirus(HRV)infection in influenza-like Illness(ILIs)patients in Mianyang,Sichuan province,China.Methods Throat swabs were collected from patients of ILIs in sentinel hospitals in Mianyang during 2021-2022.Real-time fluorescence quantitative PCR was used to detect 16 common pathogens.The VP4/VP2 coding region genes of HRV positive samples were amplified by nest PCR.The phylogeny,consistency and amino acid variation of different serotypes were analyzed and compared with reference sequences from GenBank database.Results A total of 332 ILIs'samples were collected with a virus detection rate of 58.73％(195/332)in Mianyang.Among them,23 samples(23/332)were HRV-positive,and 18 VP4/VP2 sequences of HRV strains were successfully amplified.It was found that 13 HRV serotypes were detected in ILIs samples in Mianyang,which belonged to three genotypes,namely HRV-A(12 strains),HRV-B(5 strains)and HRV-C(1 strain).Conclusions HRV was one of the pathogens of ILIs cases in Mianyang during 2021-2022,with HRV-A types as the dominant strains.
关键词
流感样病例/鼻病毒/病原特征/遗传进化Key words
Influenza-like cases/rhinovirus/pathogenic characterization/genetic evolution引用本文复制引用
基金项目
国家病原微生物资源库项目(NPRC-32)
国家重点研发计划(2023YFC2308204)
出版年
2024
NETL
NSTL
万方数据