摘要
目的 了解新型冠状病毒(2019 novel coronavirus,2019-nCoV)阳性病例中人鼻病毒(human rhinovirus,HRV)的共感染特征及系统进化特征.方法 使用实时荧光定量PCR(real-time fluorescence quantitative polymerase chain reaction,qPCR)对 7 213 份 2019-nCoV 核酸阳性样本进行了包括HRV在内的10种常见呼吸道病毒检测,分析其共感染特征;对HRV核酸阳性样本进行了 VP4/VP2基因片段测序并构建进化树,分析其系统进化特征.结果 2019-nCoV核酸阳性样本中合并HRV感染最多,为97例,阳性率1.34％;其次是普通冠状病毒(coronavirus,CoV),为36例,阳性率0.50％.HRV共感染率与其他病毒相比,差异具有统计学意义(x2=318.09,P＜0.001).18岁以下组HRV共感染率最高,差异具有统计学意义(x2=36.77,P＜0.001).共感染HRV未呈现明显季节性.其中39株HRV基因片段测序成功(39/97,40.21％),进化树分析表明,10株被划分到HRV-A组,9株被划分到HRV-B组,20株被划分到HRV-C组.39份样本共检测到17个血清型,其中B6和C15为优势血清型,分别为6株(HRV-B中占比66.67％)和14株(HRV-C中占比70％),其他血清型呈散在分布.结论 HRV是2019-nCoV核酸阳性病例中共感染率最高的病毒类呼吸道病原体,共感染率在18岁以下组最高.杭州地区2019-nCoV阳性病例中共感染的HRV-A组、HRV-B组和HRV-C组均有发现,血清型呈现多样性.
Abstract
Objective To investigate the co-infection and phylogenetic analysis of human rhinovirus(HRV)in 2019 novel coronavirus(2019-nCoV)positive samples.Methods Ten common respiratory viruses,including HRV were detected by real-time fluorescence quantitative polymerase chain reaction(qPCR)in 7 213 samples of 2019-nCoV positive cases and the co-infection characteristics were analyzed.The VP4/VP2 gene fragment of HRV was amplified and sequenced.Phylogenetic trees were constructed.Results HRV accounted for 1.34％of the 2019-nCoV positive samples(97/7 213),followed by common coronavirus(0.50％,36/7 213).The co-infection rate of HRV in 2019-nCoV positive samples was significantly different from that of other viruses(x2=318.09,P＜0.001).There was significant difference in HRV co-infection rate among different age groups(x2=36.77,P＜0.001),the peak was in＜18 years age group.The co-infection rate of HRV had no significant difference in different seasons.The VP4/VP2 gene fragments of 39 HRV strains(40.21％,39/97)were successfully sequenced and made phylogenetic analysis.There were 10 strains of HRV-A,9 strains of HRV-B and 20 strains of HRV-C.Seventeen subtypes were identified,of which B6(66.67％,6/9)and C15(70％,14/20)were the most prevalent and other subtypes were scattered.Conclusions The co-infection rate of HRV in patients with 2019-nCoV infection was the highest.The highest co-infection rate was in＜18 years age group.Group A,B,and C of HRV were found in 2019-nCoV positive samples,and serotypes present diversity.
NETL
NSTL
万方数据