2019-2020年冬春季麻城市社区季节性流感病毒感染率血清学队列研究
A community-based serological cohort study on incidence of seasonal influenza virus infection in Macheng city from winter 2019 to spring 2020
范津菘 1占建波 2陈悦 1董少波 3鲁健 1郭俊峰 1林小靖 1蓝雨 1秦堃 1周剑芳 1胡兵 2徐翠玲1
作者信息
- 1. 中国疾病预防控制中心病毒病预防控制所,北京 102206
- 2. 湖北省疾病预防控制中心,武汉 430000
- 3. 麻城市疾病预防控制中心,麻城 438300
- 折叠
摘要
目的 确定社区内有血清学证据的各亚型/系季节性流感病毒感染率,分析影响季节性流感病毒感染的因素.方法 在2019年11月(即流感流行季节前)麻城市15个村/街道招募6～59岁居民建立队列,开展横断面基线调查,采集血清,收集人口学及儿童防护知识、行为、态度等信息,在次年7月(即流感流行季节后)进行横断面随访调查,再次采集血清.两次调查配对血清通过血凝抑制(haemagglutination inhibition,HI)试验或微量中和(micro-neutralisation,MN)试验,使用各亚型/系流感病毒流行代表株作为抗原,测定流感病毒特异性抗体,如果抗体有四倍或以上增高,确定为感染.运用单因素和多因素Logistic回归分析感染A(H3N2)、B/Victoria流感病毒的影响因素.结果 2019年11月,队列纳入800名研究对象,包括340名6～17岁儿童和460名18～59岁成年人,2020年7月随访到605名研究对象(包括224名儿童、381名成人)并获得了流行季节前后配对血清.25.3％(153/605)的研究对象至少感染过一种亚型/系的季节性流感病毒.儿童各亚型/系流感病毒总感染率为44.2％(95％CI:37.6％～50.8％)显著高于成人的感染率14.1％(95％CI:10.7％～17.7％).儿童的A(H3N2)流感病毒感染率最高,其次为B/Victoria.流感流行季节前A(H3N2)[OR=0.88(95％CI:0.84～0.93)]、B/Victoria[OR=0.97(95％CI:0.95～0.99)]病毒的 MN 或 HI 抗体水平与儿童是否感染该亚型/系流感病毒相关.结论 2019-2020冬春季节麻城市6～59岁居民季节性流感病毒感染率较高,近50％的6～17岁儿童感染过季节性流感病毒.流行季节前较高的HI/MN抗体水平可能降低A(H3N2)和B/Victoria流感病毒的感染风险.
Abstract
Objective To determine incidence of seasonal influenza virus infection in the community and to analyze the factors influencing seasonal influenza virus infection.Methods This study recruited residents aged 6-59 years to build a cohort in 15 villages/streets in Macheng city in November 2019.Meanwhile,a cross-sectional baseline survey was conducted immediately to collect sera,information on demographics and child protection knowledge,behaviors,as well as attitudes using a questionnaire from the participants enrolled in the cohort(i.e.,before the influenza epidemic season).In July 2020,a cross-sectional follow-up survey was conducted to collect sera once again(i.e.,after the influenza season).Paired sera from the two cross-sectional surveys were tested for influenza virus-specific antibodies by hemagglutination inhibition(HI)test or micro-neutralization(MN)test using a circulating representative strain of each subtype/lineage of influenza virus as the test antigen.The infections with influenza virus subtype/lineage was confirmed if there was a four-fold or more increase in titers of antibodies against circulating representative strain of the subtype/lineage of influenza virus.Factors influencing infection with influenza A(H3N2)and B/Victoria viruses were analyzed using univariable and multivariable logistic regression.Results In November 2019,800 study participants were enrolled in the cohort,including 340 children aged 6-17 years and 460 adults aged 18-59 years;605 study participants(including 224 children and 381 adults)were followed up in July 2020 and their paired sera were obtained before and after the influenza season.25.3％(153/605)of the participants were confirmed to be infected with at least one subtype/lineage of seasonal influenza virus by HI and MN tests.The overall incidence of influenza viruses of all subtypes/lineages in children was 44.2％(95％CI:37.6％-50.8％)which was significantly higher than the incidence of 14.1％in adults(95％CI:10.7％-17.7％).Children had the highest incidence of influenza A(H3N2)virus infection,followed by B/Victoria.MN or HI antibody titers in A(H3N2)[OR=0.88(95％CI:0.84-0.93)]and B/Victoria[OR=0.97(95％CI:0.95-0.99)]before the influenza season were significantly associated with whether children were infected with that subtype/lineage of influenza virus.Conclusions The residents aged 6-59 years in Macheng city had a substantial incidence of seasonal influenza virus infection during the influenza season from winter 2019 to spring 2020.Notably,almost half of children aged 6-17 years have been infected with seasonal influenza virus.Higher titers of HI/MN antibodies against seasonal influenza virus before the influenza season would be likely to reduce the risk of infection with influenza A(H3N2)and B/Victoria.
关键词
季节性流感/队列研究/血凝抑制试验/抗体滴度Key words
Seasonal influenza/Cohort study/Hemagglutination inhibition test/Antibody titer引用本文复制引用
基金项目
国家自然科学基金面上项目(81974487)
国家传染病防治科技重大专项(2018ZX10201002-008-001)
国家传染病防治科技重大专项(2018ZX10201002-008-005)
出版年
2024
NETL
NSTL
万方数据