ddPCR在儿童异基因造血干细胞移植后EB病毒感染监测中的初步应用
Exploratory use of ddPCR in monitoring Epstein-Barr virus infection following allogeneic hematopoietic stem cell transplantation in children
迪拉热·迪力木拉提 1艾军红 1田娇 1刘梦佳 1王然 1谢正德1
作者信息
- 1. 国家儿童医学中心首都医科大学附属北京儿童医院北京市儿科研究所感染与病毒研究室中国医学科学院儿童危重感染诊治创新单元国家呼吸系统疾病临床医学研究中心儿科重大疾病研究教育部重点实验室儿童呼吸道感染性疾病研究北京重点实验室,北京 100045
- 折叠
摘要
目的 本研究应用微滴式数字PCR(droplet digital PCR,ddPCR)检测儿童异基因造血干细胞移植术(allogeneic hematopoietic stem cell transplantation,alloHSCT)术后 EB 病毒(Epstein-Barr virus,EBV)感染的情况,初步探讨其在临床中作为alloHSCT移植术后EBV感染监测的补充检测方法的可行性.方法 收集47例alloHSCT术后儿童共290份外周血样本,使用ddPCR和实时定量PCR(real-time quantitative PCR,qPCR)分别检测血浆中EBV DNA载量,比较两者的检出效能.通过对其中39例患儿进行连续检测,观察EBV DNA载量的动态变化,并分析两种方法的优劣势.结果 ddPCR的EBV阳性检出率显著高于qPCR(x2=20.25,P＜0.001),尤其在低病毒载量样本中表现更佳.在连续监测EBV DNA期间,39例患儿中有14例出现ddPCR阳性结果,其中有2例在ddPCR检测阳性、qPCR检测阴性的同时出现了皮疹和发热,提示ddPCR在早期发现EBV感染方面更为敏感.结论 ddPCR在alloHSCT术后患儿EBV感染监测中具有一定优势,尤其是对于低病毒载量的样本.然而,由于该技术在临床应用中尚处于探索阶段,仍需更多的研究和实践来证实ddPCR的应用价值.
Abstract
Objective This study aimed to introduce droplet digital PCR(ddPCR)for monitoring Epstein-Barr virus(EBV)infection after allogeneic hematopoietic stem cell transplantation(alloHSCT)in children and assess its viability as a complementary detection method in clinical settings.Methods A total of 290 blood samples from 47 children undergoing alloHSCT were collected.Both ddPCR and real-time quantitative PCR(qPCR)were employed to detect EBV DNA load in plasma,with a comparison of detection efficiencies between the two methods.Continuous monitoring of 39 children was conducted to observe dynamic changes in EBV DNA load in plasma and analyze the merits and drawbacks of both methods.Results The EBV positive detection rate of ddPCR was significantly higher than that of qPCR(x2=20.25,P＜0.001),particularly in samples with low viral loads.Among the children monitored continuously for EBV DNA,14 out of 39 exhibited positive ddPCR result.Notably,in two cases where patients displayed rash and fever symptoms with positive ddPCR result but negative qPCR result,ddPCR demonstrated heightened sensitivity in early EBV infection detection.Conclusions ddPCR holds certain advantages in monitoring EBV infection post-alloHSCT in children,especially for samples with low viral loads.However,as this method is still in the exploratory stage of clinical application,further research and practice are needed to validate its utility.
关键词
微滴式数字PCR/EB病毒/异基因造血干细胞移植Key words
Droplet digital PCR/Epstein-Barr virus/Allogeneic hematopoietic stem cell transplantation引用本文复制引用
基金项目
中国医科院医学与健康科技创新工程项目(2019-12M-5-026)
首都卫生发展科研专项(2024-4-1142)
北京市卫生健康委改革与发展项目()
出版年
2024
NETL
NSTL
万方数据