数字PCR在隐匿性乙肝病毒感染检测中的研究进展
Research progress in digital PCR for the detection of occult hepatitis B virus infection
张晨 1黄象艳2
作者信息
- 1. 山东第二医科大学医学检验学院,潍坊 261053;解放军联勤保障部队第九六〇医院输血医学科,济南 250031
- 2. 解放军联勤保障部队第九六〇医院输血医学科,济南 250031
- 折叠
摘要
乙型肝炎病毒(HBV)感染是全球范围内一项重大的公共卫生问题.隐匿性乙型肝炎病毒感染(OBI)是HBV感染的一种特殊类型,病毒载量极低,对输血安全构成潜在威胁,同时也为临床诊断带来巨大挑战.目前,检测肝脏和/或血液中的HBV DNA是诊断OBI的有效方法.在HBV DNA检测中,与荧光定量PCR(qPCR)相比,数字PCR(dPCR)提供了一个更加高效的检测平台,是一种用于定量极低水平核酸的可靠方法,在OBI检测中展示出十分良好的应用前景.本文主要综述了OBI的常用检测方法以及dPCR的原理和优势,概述了 dPCR在OBI检测中的应用现状.以期dPCR在OBI检测中可以充分发挥其优势,未来能够更好地服务于献血者检测和临床诊断.
Abstract
Hepatitis B virus(HBV)infection is a major public health problem worldwide.Occult hepatitis B virus infection(OBI)is a special type of HBV infection with extremely low viral load,posing a potential threat to transfusion safety and great challenges for clinical diagnosis.Currently,detecting HBV DNA in the liver and/or blood is an effective method for diagnosis of OBI.In HBV DNA detection,compared with fluorescence quantitative PCR(qPCR),digital PCR(dPCR)provides a more efficient detection platform and is a reliable method for quantifying extremely low levels of nucleic acids.It has shown great application prospects in OBI detection.This article mainly reviews the common detection method for OBI,as well as the principles and advantages of dPCR,and outlines the current application status of dPCR in OBI detection.In the hope that dPCR can fully leverage its advantages in OBI detection and better serve the detection of blood donors and clinical diagnosis in the future.
关键词
隐匿性乙肝病毒感染/数字PCR/荧光定量PCR/输血安全Key words
Occult hepatitis B virus infection/Digital PCR/Fluorescence quantitative PCR/Blood transfusion safety引用本文复制引用
基金项目
山东省自然科学基金(ZR2020MH325)
出版年
2024
NETL
NSTL
万方数据