Investigation of mosquito-borne arboviruses in the southeastern part of Gansu province in 2023
Objective To investigate the species and distribution of mosquito-borne arboviruses in the southeastern part of Gansu province.Methods In 2023,mosquitoes were collected using ultraviolet lights in Longnan and Tianshui regions of Gansu province.After classification by morphology,about 50 are only 1 batch.RT-qPCR was used to detect the RNA of Japanese encephalitis virus(JEV),Banna virus(BAV),Getah virus(GETV),Culex flavivirus(CxFV)and Tahyna virus(TAHV).The genomes of the positive samples were sequenced and the genetic evolution of the virus genomes were analyzed by bioinformatics software.Results In total,there were 8 176 mosquitoes from 4 genera and 9 species collected from Longnan and Tianshui from June to August 2023.Culex tritaeniorhynchus was the most common species with 55.80%(4 562/8 176)of the total mosquitoes collected,followed by 16.43%(1 343/8 176)of Culex pipiens pallens.A total of 263 batches of samples were obtained,and the nucleic acid test showed that 1 batch was positive for JEV,2 batches were positive for BAV,3 batches were positive for GETV and 25 batches were positive for CxFV.Conclusions Culex tritaeniorhynchus was the dominant mosquito species in the southeastern part of Gansu province,and local mosquitoes carried a variety of arboviruses.