Objective To detect and study the genetic evolution and biological characteristics of the first imported Zika virus(ZIKV)in a suspected case from Singapore to Sichuan Tianfu airport for inbound transfer on June 28,2024,and so to provide reference for prevention and control of ZIKV disease.Methods Serum sample was collected from the inbound transfer passenger,we detected ZIKV RNA using real-time RT-PCR method.The whole genome sequence was obtained through target next-generation sequencing(tNGS).CLC Genomics Workbench software was used for sequence analysis and splicing,and MEGA 7.0 software was used for phylogenetic tree construction.Results Real-time RT-PCR result showed that the serum sample was positive.The phylogenetic tree which was constructed based on the whole genome sequence of ZIKV indicated the Sichuan strain was located on Asian lineage.Gene homology analysis showed that the homology between Sichuan strain and Asian,African strains ranged from 84.4％-98.6％.The Sichuan strain showed unique molecular features of 139S,982V and 2634M.Conclusions The ZIKV strain in Sichuan inbound transfer passenger in June 2024 belongs to the Asian lineage,which agrees with the epidemiological investigation outcome.
维普
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