Complete genome sequence analysis of imported dengue type 1 virus in Gansu province in 2023
Objective To conduct whole-genome sequencing of two serum samples from imported dengue fever(DF)cases in Gansu province in 2023,analyze the molecular biological characteristics of the dengue virus(DENV)strains,accumulate baseline data for DF research in Gansu province,and provide a scientific basis for the prevention and control of imported epidemics.Methods Two serum samples that tested positive for DENV nucleic acid were sequenced for their entire genome using a third-generation sequencing platform.The obtained sequences were subjected to phylogenetic analysis,sequence homology analysis,amino acid sequence similarity analysis,and analysis of differences in mutation sites.Results Two complete DENV genomes were obtained through third-generation sequencing,with sequence lengths of 10 715 bp and 10 714 bp respectively.The nucleotide similarity between them was 99.11％,and the amino acid similarity was 99.59％.Homology and phylogenetic analyses indicated that both sequences belonged to DENV type 1 genotype Ⅲ and were highly homologous to isolates from Singapore,India,Nepal,and the Guangzhou city of China.The nucleotide similarity is greater than 96.91％,and the amino acid similarity is greater than 98.70％.Analysis of amino acid site differences showed that DENV-2023-GS001 and DENV-2023-GS002 had 10 and 14 mutation sites in the E protein,respectively,but the main sites related to virulence remained unchanged.Conclusions In 2023,two cases of imported DF in Gansu province were identified as genotype Ⅲ of DENV type 1.In terms of genetic evolutionary relationships,these viruses are most closely related to those from countries such as Singapore,India,and Nepal.This indicates that there remains a risk of DENV importation and transmission in Gansu province.
Dengue feverDengue virus type 1Whole genome sequencingAmino acid site analysis
万方数据
维普
