Molecular characteristics of the full-length genome of Quang Binh virus and Manglie virus in Yunnan province
Objective The complete genome sequence characteristics of Quang Binh virus(QBV)and Manglie virus(MaV)isolated from mosquitoes in Daluo Town,Menghai county.Methods Mosquitoes collected in Daluo Town,Menghai county in July 2012 were used for virus isolation.The nucleic acid was extracted from the supernatant of Aedes albopictus cell line(C6/36)showing cytopathic effect(CPE)positivity,and was sent to a company for sequencing after library construction.Phylogenetic and nucleotide/amino acid sequence similarity analysis was performed using DNAStar,Maff,and other softwares.Results RNA libraries of strains BNDL1205 and BNDL1227 yielded 67 336 692 and 61 259 266 qualified gene sequences(reads)respectively.After assembly and alignment,sequences of lengths 10 865 bp and 10 864 bp were obtained.Sequence analysis indicated that they belong to QBV,with strains BNDL1205 and BNDL1227 clustering with QBV(strain VN180)isolated from Vietnam on the same evolutionary branch,sharing nucleotide similarity of 84.2％and 84.1％,and amino acid similarity of 94.6％and 94.4％respectively.RNA library of strain BNDL1223 yielded 48 622 610 qualified reads.After assembly and alignment,three gene fragments(Contigs)matched MaV.Further merging using SeqMan produced a complete nucleotide sequence of 9 219 bp.Analysis revealed that strain BNDL1223 is closely related to MaV isolated in Yunnan in 2018,sharing nucleotide similarity of 97.8％and amino acid similarity of 99.2％.Conclusions During the investigation of arboviruses in mosquitoes in Daluo Town,Menghai county,three strains of viruses were identified:2 strains of QBV and 1 strain of MaV.Local mosquitoes play a significant role in the transmission of QBV and MaV,necessitating enhanced monitoring and detection of local vector mosquitoes.
万方数据
维普
